KLF4 transactivates TRIM29 expression and modulates keratin network

Abstract

The Krüppel-like factor 4 (KLF4) is well known to be a conserved zinc-containing transcription factor that participates in diverse biological processes such as cell proliferation and differentiation. In this study, we found KLF4 can bind specific site in the promoter of TRIM29 to transactivate its transcription, and sumoylation modification on 278 lysine site was not essential for KLF4 to transactivate TRIM29 transcription. It also was showed that KLF4 promoted cell migration when overexpressed, and knockdown of TRIM29 abrogated the migration triggered by KLF4. In addition, overexpression of KLF4 reduced the phosphorylation level of keratin 8 at 432 amino acid site. Our study demonstrated that KLF4 is an important transcription factor on regulating TRIM29 expression and modulates the keratin network.

Keywords

• TRIM29
• KLF4
• Migration
• Keratin