Effect of Phytochrome Deficiency on Photosynthesis, Light-Related Genes Expression and Flavonoid Accumulation in <i>Solanum lycopersicum</i> under Red and Blue Light
Pavel Pashkovskiy,
Mikhail Vereshchagin,
Vladimir Kreslavski,
Yury Ivanov,
Tamara Kumachova,
Andrey Ryabchenko,
Alexander Voronkov,
Anatoliy Kosobryukhov,
Vladimir Kuznetsov,
Suleyman I. Allakhverdiev
Affiliations
Pavel Pashkovskiy
K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia
Mikhail Vereshchagin
K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia
Vladimir Kreslavski
Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, Pushchino, 142290 Moscow, Russia
Yury Ivanov
K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia
Tamara Kumachova
Department Plant Physiology, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, Timiryazevskaya Street 49, 127550 Moscow, Russia
Andrey Ryabchenko
Tsitsin Main Botanical Garden, Russian Academy of Sciences, Botanicheskaya Street 4, 127276 Moscow, Russia
Alexander Voronkov
K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia
Anatoliy Kosobryukhov
Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, Pushchino, 142290 Moscow, Russia
Vladimir Kuznetsov
K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia
Suleyman I. Allakhverdiev
K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia
The effect of red (RL, 660 nm) and blue (BL, 450 nm) light on phy mutant tomato plants was studied. The rates of photosynthesis (Pn) and transpiration, the efficiency of the primary photochemical processes of photosynthesis, the contents of flavonoids and phenolic compounds, the low-molecular-weight antioxidant capacity (Trolox equivalent antioxidant capacity (TEAC)) of leaf extracts, and the expression of light-dependent genes were evaluated. Under RL, BL, and white fluorescent light (WFL), the Pn values decreased in the order: WT > phyb2 > phyaphyb2 > phyaphyb1phyb2, except for the Pn in phyb2 on BL. Phyb2 also had a larger number of stomata under BL and, as a result, it reached maximum transpiration. The noticeable accumulation of flavonoids and phenolic compounds was observed only in the phyb2 and phyaphyb2 mutants upon irradiation with BL, which agrees with the increased TEAC in the leaf extracts. We suggest that the increased antioxidant activity under PHYB2 deficiency and the maintenance of high photosynthesis under BL are based on an increase in the expression of the early signaling transcription factors genes BBX, HY5. The largest decrease in the content of flavonoids and TEAC was manifested with a deficiency in PHYB1, which is probably the key to maintaining the antioxidant status in BL plants.
