Infection and Drug Resistance (May 2022)
Bacteriological Quality and Antimicrobial Susceptibility Patterns Among Raw Milk Producers and Vendors in Gomole District, Borena Zone, Southern Ethiopia
Abstract
Alqeer Aliyo,1 Ayichew Seyoum,2 Zelalem Teklemariam2 1Department of Medical Laboratory Science, Institute of Health, Bule Hora University, Bule Hora, Ethiopia; 2Department of Medical Laboratory Science, College of Health and Medical Sciences Haramaya University, Harar, EthiopiaCorrespondence: Alqeer Aliyo, Department of Medical Laboratory Science, Institute of Health, Bule Hora University, Bule Hora, Ethiopia, Tel +251 968-467458, Email [email protected] and Aim: Milk consumption plays a great role in the nutrition of consumers and the income of producers as well as vendors, but their bacteriological quality causes loss of those benefits. Hence, the aim of this study was to determine the bacteriological quality, associated factors and antimicrobial susceptibility pattern among raw milk of producers and vendors in Gomole district, Borena zone, South Ethiopia, from March 1 to April 30, 2019.Methods: A cross-sectional study was conducted on 130 purposively selected study participants. Pretested structured questionnaires and observation checklists were used to collect data. Then, 15 to 20 mL of milk samples were collected from producers and vendors for laboratory analysis. Standard plate count agar and eosin methylene blue agar were used for total bacterial count and total coliform count, respectively. Bacterial isolation from poor-quality milk was performed with biochemical tests and antimicrobial susceptibility tests using Kirby Bauer’s disk diffusion method. After completeness checking, the data were analyzed by Statistical Package for the Social Sciences version 21. Chi-square (χ2) was used to analyze association factors, and a p value < 0.05 was considered statistically significant.Results: The overall means ± standard deviation of the total bacterial count (TBC) and total coliform count (TCC) were 7.75 ± 0.882 and 6.69 ± 1.545 log10 CFU/mL, respectively. The mean TBC was significantly different between producers’ and vendors’ milks t = 2.1 (P < 0.001). The proportions of raw milk TBC and TCC of poor quality were 90% and 80%, respectively. Poor hand washing practices before milking, water source and cleanliness of milk containers were associated with poor milk quality. The isolated bacteria were E. coli (30.8%), S. aureus (17.9%) and Salmonella spp. Out of the bacterial isolates, 80.4% were extensive drug resistant, 14.3% were multidrug resistant, and 5.4% were resistant against all antimicrobials used in this study.Conclusion: Ensuring proper hygienic practices during milking, storage and transportation to reduce milk contamination.Keywords: raw milk, antimicrobial susceptibility pattern, producers, vendors
