The effect of zinc doping on the cytocompatibility and antibacterial efficacy of hydroxyapatite nanoparticles for treatment of bone infection
L.A.B. Cuypers,
P. Bertsch,
R. Wang,
H.R. Harhangi,
L.S. Joziasse,
X.F. Walboomers,
L. van Niftrik,
F. Yang,
S.C.G. Leeuwenburgh
Affiliations
L.A.B. Cuypers
Radboud University Medical Center, Department of Dentistry-Regenerative Biomaterials, Philips van Leydenlaan 25, 6525 EX, Nijmegen, the Netherlands
P. Bertsch
Radboud University Medical Center, Department of Dentistry-Regenerative Biomaterials, Philips van Leydenlaan 25, 6525 EX, Nijmegen, the Netherlands
R. Wang
Radboud University Medical Center, Department of Dentistry-Regenerative Biomaterials, Philips van Leydenlaan 25, 6525 EX, Nijmegen, the Netherlands
H.R. Harhangi
Radboud University, Faculty of Science, Department of Microbiology, Heyendaalseweg 135, 6525 AJ, Nijmegen, the Netherlands
L.S. Joziasse
Radboud University Medical Center, Department of Dentistry-Regenerative Biomaterials, Philips van Leydenlaan 25, 6525 EX, Nijmegen, the Netherlands
X.F. Walboomers
Radboud University Medical Center, Department of Dentistry-Regenerative Biomaterials, Philips van Leydenlaan 25, 6525 EX, Nijmegen, the Netherlands
L. van Niftrik
Radboud University, Faculty of Science, Department of Microbiology, Heyendaalseweg 135, 6525 AJ, Nijmegen, the Netherlands
F. Yang
Radboud University Medical Center, Department of Dentistry-Regenerative Biomaterials, Philips van Leydenlaan 25, 6525 EX, Nijmegen, the Netherlands
S.C.G. Leeuwenburgh
Radboud University Medical Center, Department of Dentistry-Regenerative Biomaterials, Philips van Leydenlaan 25, 6525 EX, Nijmegen, the Netherlands; Corresponding author.
In the face of antibiotic resistance, new antibacterial methods are urgently needed for bone infections. Local delivery of therapeutic metal ions via osteogenic nanoparticles (NPs) is a viable alternative, but high concentrations risk toxicity to mammalian cells. In this study first four ions (Mg2+, Sr2+, Cu2+, Zn2+) were assessed for cytocompatibility and antibacterial properties. Only zinc ions showed antibacterial effects at non-toxic levels. Subsequently, zinc-doped hydroxyapatite nanoparticles (ZnHA NPs) were synthesized with varying zinc content (0–20 mol%). ZnHA NPs were characterized on size, morphology, composition, and antibacterial activity, with 15 mol% ZnHA NPs exhibiting the optimal balance between cytocompatibility and antibacterial efficacy. These NPs show promise as deliverable biomaterials for bone infection treatment.
