Analysis of effect of hematopoietic stem cell mobilization and collection in 67 healthy pediatric donors

Abstract

Objective To evaluate the effect of hematopoietic stem cell mobilization and collection in healthy pediatric donors. Methods 67 healthy pediatric donors were included in this study.According to the donor/recipient （D/R） body weight ratio （BWR）， they were divided into three groups： D/R BWR<1 group（n = 24）， D/R BWR 1-2 group（n = 29）， and D/R BWR>2 group （n = 14）. The mobilization， collection effect and adverse reactions of bone marrow and peripheral blood stem cells were compared among all groups. Results The quantity of mononuclear cells （MNCs） of bone marrow stem cells collected in the D/R BWR<1 group was significantly lower than those in the D/R BWR 1-2 and D/R BWR>2 group （both P < 0.001）. There was no significant difference in the quantity of bone marrow CD34+ cells among three groups （all P > 0.05）. The quantity of MNCs of peripheral blood stem cells in the D/R BWR>2 group was significantly higher than those in the D/R BWR<1 and D/R BWR 1-2 group （both P < 0.05）， and the quantity of CD34+ cells of peripheral blood stem cells in the D/R BWR>2 group was significantly higher than that in the D/R BWR<1 group （P = 0.032）. Adverse reactions were tolerated in all donors during stem cell mobilization and collection. The bone marrow and peripheral blood stem cells collected from pediatric donors could meet the transplantation needs. No poor implantation or implantation failure occurred in the related recipients during follow-up. Conclusions Hematopoietic stem cell collection from healthy pediatric donors is effective and safe. Although the quantity of stem cells collected in low D/R BWR is less compared to that in the high D/R BWR， the collected stem cells can meet the transplantation needs.

Keywords

• |pediatric donor|hematopoietic stem cell mobilization|hematopoietic stem cell collection|safety