Effects of high glucose on osteogenic differentiation of hBMSC

Abstract

Objective To investigate the effects of different glucose concentration on the proliferation and osteogen⁃ ic differentiation of human bone marrow mesenchymal stem cells (hBMSC) in vivo. Methods Cultured with basal medi⁃ um containing different glucose concentrations, CCK⁃8 cell proliferation was detected at 1, 4, 7, 10 days. The osteogenic differentiation of human bone marrow mesenchymal stem cells was observed at 7 d, which was induced by osteogenic differentiation medium with different concentration of glucose. The expressions of alkaline phosphatase (ALP), osteocal⁃ cin (OC) and collagen type I (Col⁃1) gene were detected by real⁃time fluorescence quantitative PCR. Mineralized nodule formation was displayed by calciumalizarin red staining on the seventh day. Results 10 mM glucose stimulated prolif⁃ eration of hBMSC, while the higher (＞30 mM) inhibited the proliferation (P < 0.05); Osteogenic induction can induce osteogenic differentiation of hBMSC, but the increase of glucose concentration will decrease the formation of mineralized nodules of hBMSC, inhibit the expression of osteogenic marker genes ALP, OC and Col⁃1 (P < 0.05). Conclusion The expression of Col⁃1, ALP and OC in osteoblast was down⁃regulated by high glucose, and the hBMSC proliferation was in⁃ hibited. At the same time, high glucose can reduce the osteogenic mineralization ability of stem cells and indirectly af⁃ fect bone formation and metabolism.

Keywords

• Diabetes mellitus，
• Osteointegration，
• Dental implants，
• Osteogenic differentiation，
• hBMSC，