A hyperspectral microscope based on an ultrastable common-path interferometer

Abstract

We introduce a wide field hyperspectral microscope using the Fourier-transform approach. The interferometer is based on the translating-wedge-based identical pulses encoding system, a common-path birefringent interferometer which combines compactness, intrinsic interferometric delay precision, long-term stability, and insensitivity to vibrations. We describe two different implementations of our system, which maximize fringe visibility and phase invariance over the field of view, respectively. We also demonstrate that our system can be installed as an add-on in a commercial microscope. We show high-quality hyperspectral fluorescence microscopy from stained cells and powders of inorganic pigments in the spectral range from 400 to 1100 nm, proving that our device is suited to biology and materials science. We also introduce an acquisition method that synthesizes a tunable spectral filter, providing band-passed images with the measurement of only two maps.