The Effect of Alcoholic Extract of Anethum graveolens Seed on the Changes of Testis Tissue, Sperm Parameters in Hypercholesterolemic Male Rats

Abstract

Abstract Background: For treating hypercholesterolemic in traditional medicine, Anethum graveolens seeds are used that reduce blood glucose, cholesterol and triglyceride. The aim of this study is to investigate the effect of hydroalcoholic extract of Anethum graveolens seed on the changes of testis tissue, sperm parameters in hypercholesterolemic male rats. Materials and Methods: In this study, 30 adult male rats were randomly divided into 5 groups of 6: control, hypercholesterolemic, hypercholesterolemic +alcoholic extract of Anethum graveolens seed 500 mg/kg/day, hypercholesterolemic +alcoholic extract of Anethum graveolens seed 300 mg/kg/day, healthy+alcoholic extract of Anethum graveolens seed 500 mg/kg/day. After treatment for 45 days, rats were weighed and after the dissection, sperm samples were collected from the tail epididymal and sperm parameters were studied. The testicular specimens were transferred to formalin and stained with hematoxylin-eosin. Data were analyzed using one-way ANOVA and Turkey's post- hoc tests and significant level (p<0.05) was considered. Results: In this research, in the hypercholesterolemic rats, the testicular weight was increased, but the diameter of the semnifer tubes, tubal differentiation and spermiogenese, and sperm viability were decreased compared to control (p<0.05), but in hypercholesterolemic treatment with Anethum graveolens seed these parameters were improved. Conclusion: According to the results of this study, Anethum graveolens seed has positive effects on testicular tissue and sperm parameters in hypercholesterolemic mice.

Keywords

• Anethum graveolens
• Hypercholesterolemia
• Male rat
• Sperm parameters