One health approach to study human health risks associated with Dermanyssus gallinae mites
Pavle Banović,
Angélique Foucault-Simonin,
Luka Papić,
Sara Savić,
Aleksandar Potkonjak,
Aleksandar Jurišić,
Marko Radenković,
Dragana Mijatović,
Verica Simin,
Ivana Bogdan,
Zbigniew Zając,
Joanna Kulisz,
Aneta Woźniak,
David Hartmann,
Jan Perner,
Alejandra Wu-Chuang,
Lourdes Mateos-Hernandez,
Sara Moutailler,
Alejandro Cabezas-Cruz
Affiliations
Pavle Banović
Clinic for Lyme Borreliosis and Other Tick-Borne Diseases, Department of Prevention of Rabies and Other Infectious Diseases, Pasteur Institute Novi Sad, Novi Sad, 21000, Serbia; Department of Microbiology with Parasitology and Immunology, Faculty of Medicine, University of Novi Sad, Novi Sad, 21000, Serbia; Diagnostics and Laboratory Research Task Force, Balkan Association for Vector-Borne Diseases, 21000, Novi Sad, Serbia; Corresponding author. Clinic for Lyme Borreliosis and Other Tick-Borne Diseases, Department of Prevention of Rabies and Other Infectious Diseases, Pasteur Institute Novi Sad, Novi Sad, 21000, Serbia.
Angélique Foucault-Simonin
ANSES, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France
Luka Papić
Veterinary clinic “Darvin”, Bate Brkića 32, Novi Sad, 21000, Serbia
Sara Savić
Diagnostics and Laboratory Research Task Force, Balkan Association for Vector-Borne Diseases, 21000, Novi Sad, Serbia; Scientific Veterinary Institute “Novi Sad”, 21000, Novi Sad, Serbia
Aleksandar Potkonjak
Diagnostics and Laboratory Research Task Force, Balkan Association for Vector-Borne Diseases, 21000, Novi Sad, Serbia; Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, 21000, Novi Sad, Serbia
Aleksandar Jurišić
Diagnostics and Laboratory Research Task Force, Balkan Association for Vector-Borne Diseases, 21000, Novi Sad, Serbia; Department for Environmental and Plant Protection, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia
Marko Radenković
Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 3, 21000, Novi Sad, Serbia
Dragana Mijatović
Diagnostics and Laboratory Research Task Force, Balkan Association for Vector-Borne Diseases, 21000, Novi Sad, Serbia; Department for Research & Monitoring of Rabies & Other Zoonoses, Pasteur Institute Novi Sad, 21000, Novi Sad, Serbia
Verica Simin
Diagnostics and Laboratory Research Task Force, Balkan Association for Vector-Borne Diseases, 21000, Novi Sad, Serbia; Department of Microbiology, Pasteur Institute Novi Sad, 21000, Novi Sad, Serbia
Ivana Bogdan
Diagnostics and Laboratory Research Task Force, Balkan Association for Vector-Borne Diseases, 21000, Novi Sad, Serbia; Department of Microbiology, Pasteur Institute Novi Sad, 21000, Novi Sad, Serbia
Zbigniew Zając
Department of Biology and Parasitology, Medical University of Lublin, Radziwiłłowska 11, 20-080, Lublin, Poland
Joanna Kulisz
Department of Biology and Parasitology, Medical University of Lublin, Radziwiłłowska 11, 20-080, Lublin, Poland
Aneta Woźniak
Department of Biology and Parasitology, Medical University of Lublin, Radziwiłłowska 11, 20-080, Lublin, Poland
David Hartmann
Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005, Ceske Budejovice, Czech Republic
Jan Perner
Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005, Ceske Budejovice, Czech Republic
Alejandra Wu-Chuang
ANSES, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France
Lourdes Mateos-Hernandez
ANSES, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France
Sara Moutailler
ANSES, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France
Alejandro Cabezas-Cruz
ANSES, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France; Corresponding author.
Despite the significant health risks associated with Dermanyssus gallinae infestations in humans, they are often overlooked. This study investigated a household case of D. gallinae infestation and explored the resulting clinical manifestations and risk of infection in family members. Microfluidic PCR was employed for high-throughput screening of pathogens in collected mites and blood samples from both chickens and family members. Morphological and molecular examinations confirmed the identity of the mites as D. gallinae sensu stricto (s.s.), with evidence indicating recent blood feeding. Results indicated that the mites exclusively harbored various pathogens, including Bartonella spp., Ehrlichia spp., Apicomplexa, and Theileria spp. Blood samples from family members and poultry tested negative for these pathogens, suggesting a potential reservoir role for D. gallinae. The study further identified haplotypes of D. gallinae, classifying them into D. gallinae s.s., cosmopolitan haplogroup A. Serological analysis revealed elevated IgE seroreactivity against mite proteins in the family member with bite lesions. Antibodies against Bartonella spp. were detected in this individual, indicating exposure to the pathogen. In summary, this study sheds light on the clinical manifestations, pathogen detection, and genetic characterization of D. gallinae infestations, underscoring the necessity of adopting comprehensive approaches to manage such infestations effectively.
