In Vitro Anti-Epstein Barr Virus Activity of <i>Olea europaea</i> L. Leaf Extracts
Ichrak Ben-Amor,
Bochra Gargouri,
Hamadi Attia,
Khaoula Tlili,
Imen Kallel,
Maria Musarra-Pizzo,
Maria Teresa Sciortino,
Rosamaria Pennisi
Affiliations
Ichrak Ben-Amor
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno Alcontres, 31, 98166 Messina, Italy
Bochra Gargouri
Unit of Biotechnology and Pathologies, Higher Institute of Biotechnology of Sfax, University of Sfax, Sfax 3029, Tunisia
Hamadi Attia
Unit of Biotechnology and Pathologies, Higher Institute of Biotechnology of Sfax, University of Sfax, Sfax 3029, Tunisia
Khaoula Tlili
Unit of Biotechnology and Pathologies, Higher Institute of Biotechnology of Sfax, University of Sfax, Sfax 3029, Tunisia
Imen Kallel
Laboratoire de Recherche Toxicologie-Microbiologie Environnementale et Santé, Faculté des Sciences de Sfax, Sfax 3000, Tunisia
Maria Musarra-Pizzo
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno Alcontres, 31, 98166 Messina, Italy
Maria Teresa Sciortino
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno Alcontres, 31, 98166 Messina, Italy
Rosamaria Pennisi
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno Alcontres, 31, 98166 Messina, Italy
Olea europaea L. var. sativa (OESA) preparations are widely used in traditional medicine in the Mediterranean region to prevent and treat different diseases. In this research, olive extracts derived from the leaves of the OESA tree have been screened for antioxidant activity by two methods: the DPPH free radical scavenging assay (DPPH) and the Ferric reducing antioxidant power (FRAP) assay. The DPPH assay showed that OESA possesses a stronger antioxidant activity (84%) at 1 mg/mL while the FRAP method showed a strong metal ion chelating activity (90%) at 1 mg/mL. The low IC50 values, obtained by two different methods, implies that OESA has a noticeable effect on scavenging free radicals comparable to standards. During EBV infection, the free radicals increased triggering lipid oxidation. Therefore, the monitoring of the secondary lipid peroxidation products was done by measuring malonaldehyde (MDA) and conjugated dienes (DC). The simultaneous treatment of Raji cells with OESA and TPA, as an inductorof the lytic cycle, generated a significant decrease in MDA levels and DC (p p < 0.0001). This suggests that OESA treatment has a protective effect against EBV lytic cycle induction.
