Immobilization of periodate oxidized invertase by adsorption on sepiolite

Abstract

Periodate oxidized invertase was immobilized by adsorption on sepiolite. The obtained immobilized enzyme was more resistant to washing out by concentrated salt solution, and had an eight times higher half-life at 60 ºC than adsorbed native invertase. In packed bed reactor 50 % conversion of 500 g/dm3 sucrose at 40 ºC and a flow rate of 1 bv/h was achieved. The specific productivity of the immobilized invertase was O.187 kg/dm3/h.

Keywords

• invertase
• periodate oxidation
• sepiolite
• immobilization
• sucrose.