Cryo-EM snapshots of a native lysate provide structural insights into a metabolon-embedded transacetylase reaction

Abstract

How is acetyl-CoA produced in the context of the endogenous, eukaryotic pyruvate dehydrogenase complex metabolon? Here the authors dissect the embedded transacetylase reaction through biochemical, cryo-EM, HADDOCKing and molecular dynamics methods.