AVALIAÇÃO MORFOLÓGICA E NUCLEAR DE OÓCITOS BOVINOS IMATUROS, OBTIDOS DE OVÁRIOS COM E SEM A PRESENÇA DE CORPO LÚTEO

Abstract

The in vitro embryo production (IVP) is a biotechnology that primarily enables the rapid increase in the number of offspring with superior genetic merit . Currently, Brazil is the world leader in IVP and much of this success is due to the extensive research carried out in that area. The selection of good quality oocytes for this technique is critical to obtaining quality embryos and satisfactory pregnancy rates. Thus, this study aimed to assess oocytes obtained from ovaries with and without the presence of corpus luteum (CL), according to the morphological and nuclear classification, in order to identify the possible influence of CL on the viability of oocytes. They were collected from slaughterhouse ovaries and separated into two groups, with and without CL. Follicles were aspirated and the oocytes were selected, ranked, and then stained with Hoechst 33342 and evaluated by epifluorescence microscope for nuclear evaluation. A descriptive analysis of each treatment with respect to morphological and nuclear classification. The results were analyzed using non-parametric tests, with the effect of the corpus luteum verified using the chisquare test (χ2) (R Core Team, 2013). It was concluded that the presence of CL does not influence the quality of immature oocytes, from the quantitative and morphological nuclear standpoint.

Keywords

• Cumulus-Oocyte complex
• IVP
• meiosis