The effect of high-polyphenol Mediterranean diet on visceral adiposity: the DIRECT PLUS randomized controlled trial
Hila Zelicha,
Nora Kloting,
Alon Kaplan,
Anat Yaskolka Meir,
Ehud Rinott,
Gal Tsaban,
Yoash Chassidim,
Matthias Bluher,
Uta Ceglarek,
Berend Isermann,
Michael Stumvoll,
Rita Nana Quayson,
Martin von Bergen,
Beatrice Engelmann,
Ulrike E. Rolle-Kampczyk,
Sven-Bastiaan Haange,
Kieran M. Tuohy,
Camilla Diotallevi,
Ilan Shelef,
Frank B. Hu,
Meir J. Stampfer,
Iris Shai
Affiliations
Hila Zelicha
Faculty of Health Sciences, The Health & Nutrition Innovative International Research Center, Ben-Gurion University of the Negev
Nora Kloting
Department of Medicine, University of Leipzig
Alon Kaplan
Faculty of Health Sciences, The Health & Nutrition Innovative International Research Center, Ben-Gurion University of the Negev
Anat Yaskolka Meir
Faculty of Health Sciences, The Health & Nutrition Innovative International Research Center, Ben-Gurion University of the Negev
Ehud Rinott
Faculty of Health Sciences, The Health & Nutrition Innovative International Research Center, Ben-Gurion University of the Negev
Gal Tsaban
Faculty of Health Sciences, The Health & Nutrition Innovative International Research Center, Ben-Gurion University of the Negev
Yoash Chassidim
Department of Engineering, Sapir Academic College
Matthias Bluher
Helmholtz Institute for Metabolic, Obesity and Vascular Research (HI-MAG) of the Helmholtz Zentrum München at the University of Leipzig and University Hospital Leipzig
Uta Ceglarek
Department of Medicine, University of Leipzig
Berend Isermann
Department of Medicine, University of Leipzig
Michael Stumvoll
Department of Medicine, University of Leipzig
Rita Nana Quayson
Department of Medicine, University of Leipzig
Martin von Bergen
Department of Medicine, University of Leipzig
Beatrice Engelmann
Department of Medicine, University of Leipzig
Ulrike E. Rolle-Kampczyk
Department of Medicine, University of Leipzig
Sven-Bastiaan Haange
Department of Medicine, University of Leipzig
Kieran M. Tuohy
Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach
Camilla Diotallevi
Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach
Ilan Shelef
Soroka University Medical Center
Frank B. Hu
Department of Epidemiology, Harvard T.H. Chan School of Public Health
Meir J. Stampfer
Department of Epidemiology, Harvard T.H. Chan School of Public Health
Iris Shai
Faculty of Health Sciences, The Health & Nutrition Innovative International Research Center, Ben-Gurion University of the Negev
Abstract Background Mediterranean (MED) diet is a rich source of polyphenols, which benefit adiposity by several mechanisms. We explored the effect of the green-MED diet, twice fortified in dietary polyphenols and lower in red/processed meat, on visceral adipose tissue (VAT). Methods In the 18-month Dietary Intervention Randomized Controlled Trial PoLyphenols UnproceSsed (DIRECT-PLUS) weight-loss trial, 294 participants were randomized to (A) healthy dietary guidelines (HDG), (B) MED, or (C) green-MED diets, all combined with physical activity. Both isocaloric MED groups consumed 28 g/day of walnuts (+ 440 mg/day polyphenols). The green-MED group further consumed green tea (3–4 cups/day) and Wolffia globosa (duckweed strain) plant green shake (100 g frozen cubes/day) (+ 800mg/day polyphenols) and reduced red meat intake. We used magnetic resonance imaging (MRI) to quantify the abdominal adipose tissues. Results Participants (age = 51 years; 88% men; body mass index = 31.2 kg/m2; 29% VAT) had an 89.8% retention rate and 79.3% completed eligible MRIs. While both MED diets reached similar moderate weight (MED: − 2.7%, green-MED: − 3.9%) and waist circumference (MED: − 4.7%, green-MED: − 5.7%) loss, the green-MED dieters doubled the VAT loss (HDG: − 4.2%, MED: − 6.0%, green-MED: − 14.1%; p < 0.05, independent of age, sex, waist circumference, or weight loss). Higher dietary consumption of green tea, walnuts, and Wolffia globosa; lower red meat intake; higher total plasma polyphenols (mainly hippuric acid), and elevated urine urolithin A polyphenol were significantly related to greater VAT loss (p < 0.05, multivariate models). Conclusions A green-MED diet, enriched with plant-based polyphenols and lower in red/processed meat, may be a potent intervention to promote visceral adiposity regression. Trial registration ClinicalTrials.gov , NCT03020186