Fabrication of Sericin/Agrose Gel Loaded Lysozyme and Its Potential in Wound Dressing Application
Meirong Yang,
Yejing Wang,
Gang Tao,
Rui Cai,
Peng Wang,
Liying Liu,
Lisha Ai,
Hua Zuo,
Ping Zhao,
Ahmad Umar,
Chuanbin Mao,
Huawei He
Affiliations
Meirong Yang
State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China
Yejing Wang
State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China
Gang Tao
State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China
Rui Cai
College of Biotechnology, Southwest University, Beibei, Chongqing 400715, China
Peng Wang
College of Biotechnology, Southwest University, Beibei, Chongqing 400715, China
Liying Liu
State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China
Lisha Ai
State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China
Hua Zuo
College of Pharmaceutical Sciences, Southwest University, Beibei, Chongqing 400715, China
Ping Zhao
State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China
Ahmad Umar
Department of Chemistry, College of Science and Arts and Promising Centre for Sensors and Electronics Devices (PCSED), Najran University, P.O. Box 1988, Najran 11001, Saudi Arabia
Chuanbin Mao
Department of Chemistry & Biochemistry, Stephenson Life Science Research Center, University of Oklahoma, 101 Stephenson Parkway, Norman, OK 73019, USA
Huawei He
State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China
Sericin is a biomaterial resource for its significant biodegradability, biocompatibility, hydrophilicity, and reactivity. Designing a material with superabsorbent, antiseptic, and non-cytotoxic wound dressing properties is advantageous to reduce wound infection and promote wound healing. Herein, we propose an environment-friendly strategy to obtain an interpenetrating polymer network gel through blending sericin and agarose and freeze-drying. The physicochemical characterizations of the sericin/agarose gel including morphology, porosity, swelling behavior, crystallinity, secondary structure, and thermal property were well characterized. Subsequently, the lysozyme loaded sericin/agarose composite gel was successfully prepared by the solution impregnation method. To evaluate the potential of the lysozyme loaded sericin/agarose gel in wound dressing application, we analyzed the lysozyme loading and release, antimicrobial activity, and cytocompatibility of the resulting gel. The results showed the lysozyme loaded composite gel had high porosity, excellent water absorption property, and good antimicrobial activities against Escherichia coli and Staphylococcus aureus. Also, the lysozyme loaded gel showed excellent cytocompatibility on NIH3T3 and HEK293 cells. So, the lysozyme loaded sericin/agarose gel is a potential alternative biomaterial for wound dressing.
