Molecular mechanism of toxin neutralization in the HipBST toxin-antitoxin system of Legionella pneumophila

Xiangkai Zhen; Yongyu Wu; Jinli Ge; Jiaqi Fu; Le Ye; Niannian Lin; Zhijie Huang; Zihe Liu; Zhao-qing Luo; Jiazhang Qiu; Songying Ouyang

doi:10.1038/s41467-022-32049-x

Nature Communications (Jul 2022)

Molecular mechanism of toxin neutralization in the HipBST toxin-antitoxin system of Legionella pneumophila

Xiangkai Zhen,
Yongyu Wu,
Jinli Ge,
Jiaqi Fu,
Le Ye,
Niannian Lin,
Zhijie Huang,
Zihe Liu,
Zhao-qing Luo,
Jiazhang Qiu,
Songying Ouyang

Affiliations

Xiangkai Zhen: Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal University
Yongyu Wu: Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal University
Jinli Ge: State Key Laboratory for Zoonotic Diseases, College of Veterinary Medicine, Jilin University
Jiaqi Fu: Purdue Institute for Inflammation, Immunology and Infectious Disease and Department of Biological Sciences, Purdue University
Le Ye: Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal University
Niannian Lin: Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal University
Zhijie Huang: Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal University
Zihe Liu: Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal University
Zhao-qing Luo: Purdue Institute for Inflammation, Immunology and Infectious Disease and Department of Biological Sciences, Purdue University
Jiazhang Qiu: State Key Laboratory for Zoonotic Diseases, College of Veterinary Medicine, Jilin University
Songying Ouyang: Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal University

DOI: https://doi.org/10.1038/s41467-022-32049-x
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 14

Abstract

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Here, the authors demonstrate that the Legionella pneumophila T4SS effector protein Lpg2370 is a Ser/Thr kinase and a toxin of a tripartite HipBST toxin-antitoxin (TA) system. Structural data and biochemical analysis provide detailed insights into the toxin neutralization mechanism in the HipBST TA.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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