Communications Biology (Feb 2022)

Identification of SARS-CoV-2 inhibitors targeting Mpro and PLpro using in-cell-protease assay

  • Anoop Narayanan,
  • Manju Narwal,
  • Sydney A. Majowicz,
  • Carmine Varricchio,
  • Shay A. Toner,
  • Carlo Ballatore,
  • Andrea Brancale,
  • Katsuhiko S. Murakami,
  • Joyce Jose

DOI
https://doi.org/10.1038/s42003-022-03090-9
Journal volume & issue
Vol. 5, no. 1
pp. 1 – 17

Abstract

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The development of new drugs to treat COVID-19 continues to be of critical importance. This paper describes a new in-cell assay to screen for inhibitors of SARSCoV-2 in live cells. 8 new compounds are reported along with a crystal structure of a key viral protease, Mpro, in complex with one of the newly discovered compounds.