Long Non-coding RNA Uc.48+ Small Interfering RNA Alleviates Neuroinflammatory Hyperalgesia in Gp120-Treated Rats via the P2Y12 Receptor

Lichao Peng; Bing Wu; Bing Wu; Liran Shi; Liran Shi; Lifang Zou; Lifang Zou; Lin Li; Lin Li; Runan Yang; Runan Yang; Xiumei Xu; Xiumei Xu; Guilin Li; Guilin Li; Shuangmei Liu; Shuangmei Liu; Chunping Zhang; Chunping Zhang; Shangdong Liang; Shangdong Liang

doi:10.3389/fnins.2021.663962

Frontiers in Neuroscience (Jul 2021)

Long Non-coding RNA Uc.48+ Small Interfering RNA Alleviates Neuroinflammatory Hyperalgesia in Gp120-Treated Rats via the P2Y12 Receptor

Lichao Peng,
Bing Wu,
Bing Wu,
Liran Shi,
Liran Shi,
Lifang Zou,
Lifang Zou,
Lin Li,
Lin Li,
Runan Yang,
Runan Yang,
Xiumei Xu,
Xiumei Xu,
Guilin Li,
Guilin Li,
Shuangmei Liu,
Shuangmei Liu,
Chunping Zhang,
Chunping Zhang,
Shangdong Liang,
Shangdong Liang

Affiliations

Lichao Peng: School of Life Sciences, Xiamen University, Xiamen, China
Bing Wu: Neuropharmacology Laboratory of Physiology Department, Medical School of Nanchang University, Nanchang, China
Bing Wu: Department of Cell Biology, Medical School of Nanchang University, Nanchang, China
Liran Shi: Neuropharmacology Laboratory of Physiology Department, Medical School of Nanchang University, Nanchang, China
Liran Shi: Department of Cell Biology, Medical School of Nanchang University, Nanchang, China
Lifang Zou: Neuropharmacology Laboratory of Physiology Department, Medical School of Nanchang University, Nanchang, China
Lifang Zou: Department of Cell Biology, Medical School of Nanchang University, Nanchang, China
Lin Li: Neuropharmacology Laboratory of Physiology Department, Medical School of Nanchang University, Nanchang, China
Lin Li: Department of Cell Biology, Medical School of Nanchang University, Nanchang, China
Runan Yang: Neuropharmacology Laboratory of Physiology Department, Medical School of Nanchang University, Nanchang, China
Runan Yang: Department of Cell Biology, Medical School of Nanchang University, Nanchang, China
Xiumei Xu: Neuropharmacology Laboratory of Physiology Department, Medical School of Nanchang University, Nanchang, China
Xiumei Xu: Department of Cell Biology, Medical School of Nanchang University, Nanchang, China
Guilin Li: Neuropharmacology Laboratory of Physiology Department, Medical School of Nanchang University, Nanchang, China
Guilin Li: Department of Cell Biology, Medical School of Nanchang University, Nanchang, China
Shuangmei Liu: Neuropharmacology Laboratory of Physiology Department, Medical School of Nanchang University, Nanchang, China
Shuangmei Liu: Department of Cell Biology, Medical School of Nanchang University, Nanchang, China
Chunping Zhang: Department of Cell Biology, Medical School of Nanchang University, Nanchang, China
Chunping Zhang: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Shangdong Liang: Neuropharmacology Laboratory of Physiology Department, Medical School of Nanchang University, Nanchang, China
Shangdong Liang: Department of Cell Biology, Medical School of Nanchang University, Nanchang, China

DOI: https://doi.org/10.3389/fnins.2021.663962
Journal volume & issue: Vol. 15

Abstract

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Human immunodeficiency virus envelope glycoprotein 120 (gp120) leads to hyperalgesia. Long non-coding RNAs are characterized by the lack of a protein-coding sequence and may contribute to the development and maintenance of inflammatory and neuroinflammatory pain. Rats with neuroinflammatory pain were established by gp120 treatment, which is featured by intensified pain behaviors. Long non-coding RNA uc.48+ was increased in the dorsal root ganglia of gp120-treated rats, and small interfering RNA that targets uc.48+ markedly alleviated hyperalgesia in gp120-treated rats. Notably, uc.48+ overexpression increased P2Y12 expression in control rats dorsal root ganglia and induced hyperalgesia. Uc.48+ small interfering RNA inhibited P2Y12 expression in gp120-treated rats. Uc.48+ potentiated P2Y12 receptor functions in the neurons and heterologous cells. Therefore, uc.48+ siRNA treatment reduced the upregulation of P2Y12 expression and function in DRG neurons, and, hence, alleviated hyperalgesia in gp120-treated rats.

Published in Frontiers in Neuroscience

ISSN: 1662-4548 (Print); 1662-453X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: http://www.frontiersin.org/neuroscience

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