Clinical and Experimental Gastroenterology (Jun 2020)
Effects of Aerobic Exercise Protocol on Genes Related to Insulin Resistance and Inflammation in the Pancreas of ob/ob Mice with NAFLD
Abstract
Lucas Lucena Simões e Silva,1 Matheus Santos de Sousa Fernandes,1 Márcia Saldanha Kubrusly,2 Cynthia Rodrigues Muller,3 Anna Laura Viacava Américo,3 Jose Tadeu Stefano,1 Fabiana Sant’Anna Evangelista,3 Claudia Pinto Oliveira,1 José Jukemura4 1Division of Gastroenterology and Hepatology, Department of Gastroenterology (LIM 07), University of São Paulo School of Medicine, São Paulo, Brazil; 2Department of Experimental Pathophysiology, Faculty of Medicine, University of Sao Paulo, Sao Paulo, Brazil; 3School of Arts, Science and Humanities, University of São Paulo, São Paulo, Brazil; 4Division of Digestive Surgery - Department of Gastroenterology, São Paulo State Cancer Institute - HCFMUSP, University of Sao Paulo School of Medicine, Sao Paulo, BrazilCorrespondence: Lucas Lucena Simões e SilvaUniversity of Sao Paulo School of Medicine, Department of Gastroenterology, Av. Dr. Arnaldo, 455, São Paulo, Cerqueira Cesar 01246-903, BrazilTel +55 (11) 3061-7000Email [email protected]: To evaluate the effect of 8 weeks of aerobic training on insulin resistance and inflammatory response in obese mice (ob/ob) with NAFLD.Materials and Methods: Male ob/ob mice were randomly divided into sedentary (n=7) and trained (n=7) groups. Aerobic training consisted of 5 weekly sessions, 60 min per session at 60% of the maximum speed of the running test. Hepatic and pancreatic samples were collected to evaluate histological features and gene expression associated with insulin resistance and inflammatory response after 8-week experiment protocol. RNA was performed by TRIzol®. PCR experiments were performed using the Rotor-Gene RG-3000. Parametric data were assessed by t-test, one-way ANOVA and Bonferroni test for multiple comparisons. Non-parametric data were assessed by the Mann–Whitney tests with Dunn’s post-test of multiple comparisons. Histological analysis was assessed by chi-square test with Fisher’s exact test. Significant variables were considered when p< 0.05. All the analyses were performed by GraphPad Prism V6.0 software (GraphPad Software Inc.).Results: Reductions in bodyweight (p = 0.008), weight evolution (p = 0.03), food intake (p < 0.0001) and fat content were observed in trained group. Moreover, the trained group showed better results in peak velocity (p=0.03) physical effort tolerance (p=0.006) and distance (p=0.01). Gene expression showed differences in IL-10 (p=0.03) and GLUT-2 (p=0.03) in hepatic analysis, between groups. Pancreatic gene expression showed difference between groups in IRS-2 (p=0.004), GLUT-2 (p=0.03) and IL-10 (p=0.008) analysis. Also, the trained group showed lower values for interlobular fat and inflammatory infiltrate in histological analysis when compared to sedentary animals.Conclusion: An 8-week physical training protocol was able to attenuate bodyweight gain, food intake and generate positive effects on gene expression related to insulin resistance and inflammation in both liver and pancreas of ob/ob mice.Keywords: fatty liver, exercise, obese mice, insulin resistance, inflammation, pancreas
