The evolutionarily highly conserved MST2 suppresses WSSV infection by promoting apoptosis in shrimp

Junying Xu; Fen Li; Lin Wang; Wenyu Xue; Na Li; Xu Yang; Boshuai Liu; Yinghua Shi; Defeng Li; Chengzhang Wang; Xiaoyan Zhu; Zhentian Li; Yalei Cui

Aquaculture Reports (Oct 2022)

The evolutionarily highly conserved MST2 suppresses WSSV infection by promoting apoptosis in shrimp

Junying Xu,
Fen Li,
Lin Wang,
Wenyu Xue,
Na Li,
Xu Yang,
Boshuai Liu,
Yinghua Shi,
Defeng Li,
Chengzhang Wang,
Xiaoyan Zhu,
Zhentian Li,
Yalei Cui

Affiliations

Junying Xu: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan 450002, China
Fen Li: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan 450002, China
Lin Wang: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan 450002, China
Wenyu Xue: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan 450002, China
Na Li: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, China
Xu Yang: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan 450002, China
Boshuai Liu: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan 450002, China
Yinghua Shi: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan 450002, China; Henan Key Laboratory of Innovation and Utilization of Grassland Resources, Zhengzhou, Henan 450002, China; Henan Forage Engineering Technology Research Center, Zhengzhou, Henan 450002, China
Defeng Li: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan 450002, China; Henan Key Laboratory of Innovation and Utilization of Grassland Resources, Zhengzhou, Henan 450002, China; Henan Forage Engineering Technology Research Center, Zhengzhou, Henan 450002, China
Chengzhang Wang: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan 450002, China; Henan Key Laboratory of Innovation and Utilization of Grassland Resources, Zhengzhou, Henan 450002, China; Henan Forage Engineering Technology Research Center, Zhengzhou, Henan 450002, China
Xiaoyan Zhu: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan 450002, China; Henan Key Laboratory of Innovation and Utilization of Grassland Resources, Zhengzhou, Henan 450002, China; Henan Forage Engineering Technology Research Center, Zhengzhou, Henan 450002, China
Zhentian Li: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan 450002, China; Henan Key Laboratory of Innovation and Utilization of Grassland Resources, Zhengzhou, Henan 450002, China; Henan Forage Engineering Technology Research Center, Zhengzhou, Henan 450002, China
Yalei Cui: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan 450002, China; Henan Key Laboratory of Innovation and Utilization of Grassland Resources, Zhengzhou, Henan 450002, China; Henan Forage Engineering Technology Research Center, Zhengzhou, Henan 450002, China; Correspondence to: Henan Agricultural University, No.15 Longzihu University Area, Zhengdong New District, Zhengzhou 450046, China.

Journal volume & issue: Vol. 26
p. 101296

Abstract

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Mammalian Ste20-like kinase 2 (MST2) is a core component of the Hippo pathway and plays an important role in regulating cell apoptosis, cell proliferation and cell cycle progression, but it has rarely been studied in invertebrates, especially crustaceans. In this study, the MST2 gene in penaeus japonicus shrimp was characterized. A 2111 bp long nucleotide sequence encoding 690 amino acids was obtained. The molecular weight of MST2 protein is 78.47222 kDa. The bioinformatics analysis indicated that the MST2 protein has an S_TK-c (Ser/Thr kinase) domain and a SARAH (Salvador/Ras-association domain family/Hippo) domain, which is consistent with other species. The analysis of subcellular localization showed that MST2 was mainly present in the cytoplasm. Real time PCR and apoptosis analysis indicated that MST2 was involved in the white spot syndrome virus (WSSV) infection process and inhibited viral infection by promoting apoptosis. This study revealed the potential function of MST2 in virus invasion and cell apoptosis, providing a new target for preventing shrimp virus infection.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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