Applying Next-Generation Sequencing Platforms for Pharmacogenomic Testing in Clinical Practice

Alireza Tafazoli; Alireza Tafazoli; Henk-Jan Guchelaar; Henk-Jan Guchelaar; Wojciech Miltyk; Adam J. Kretowski; Adam J. Kretowski; Jesse J. Swen; Jesse J. Swen

doi:10.3389/fphar.2021.693453

Frontiers in Pharmacology (Aug 2021)

Applying Next-Generation Sequencing Platforms for Pharmacogenomic Testing in Clinical Practice

Alireza Tafazoli,
Alireza Tafazoli,
Henk-Jan Guchelaar,
Henk-Jan Guchelaar,
Wojciech Miltyk,
Adam J. Kretowski,
Adam J. Kretowski,
Jesse J. Swen,
Jesse J. Swen

Affiliations

Alireza Tafazoli: Department of Analysis and Bioanalysis of Medicines, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Bialystok, Bialystok, Poland
Alireza Tafazoli: Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland
Henk-Jan Guchelaar: Department of Clinical Pharmacy and Toxicology, Leiden University Medical Center, Leiden, Netherlands
Henk-Jan Guchelaar: Leiden Network of Personalized Therapeutics, Leiden, Netherlands
Wojciech Miltyk: Department of Analysis and Bioanalysis of Medicines, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Bialystok, Bialystok, Poland
Adam J. Kretowski: Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland
Adam J. Kretowski: Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, Bialystok, Poland
Jesse J. Swen: Department of Clinical Pharmacy and Toxicology, Leiden University Medical Center, Leiden, Netherlands
Jesse J. Swen: Leiden Network of Personalized Therapeutics, Leiden, Netherlands

DOI: https://doi.org/10.3389/fphar.2021.693453
Journal volume & issue: Vol. 12

Abstract

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Pharmacogenomics (PGx) studies the use of genetic data to optimize drug therapy. Numerous clinical centers have commenced implementing pharmacogenetic tests in clinical routines. Next-generation sequencing (NGS) technologies are emerging as a more comprehensive and time- and cost-effective approach in PGx. This review presents the main considerations for applying NGS in guiding drug treatment in clinical practice. It discusses both the advantages and the challenges of implementing NGS-based tests in PGx. Moreover, the limitations of each NGS platform are revealed, and the solutions for setting up and management of these technologies in clinical practice are addressed.

Published in Frontiers in Pharmacology

ISSN: 1663-9812 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://journal.frontiersin.org/journals/pharmacology

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Abstract

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