Generation of seven induced pluripotent stem cell lines from neonates of different ethnic backgrounds

Yingnan Yin; Andrew J. Petersen; Cheryl Soref; William D. Richards; Tenneille Ludwig; Seth Taapken; Erich Berndt; Su-Chun Zhang; Anita Bhattacharyya

Stem Cell Research (Jan 2019)

Generation of seven induced pluripotent stem cell lines from neonates of different ethnic backgrounds

Yingnan Yin,
Andrew J. Petersen,
Cheryl Soref,
William D. Richards,
Tenneille Ludwig,
Seth Taapken,
Erich Berndt,
Su-Chun Zhang,
Anita Bhattacharyya

Affiliations

Yingnan Yin: Waisman Center, University of Wisconsin, Madison, WI, USA
Andrew J. Petersen: Waisman Center, University of Wisconsin, Madison, WI, USA
Cheryl Soref: Waisman Center, University of Wisconsin, Madison, WI, USA
William D. Richards: Waisman Center, University of Wisconsin, Madison, WI, USA
Tenneille Ludwig: WiCell Research Institute, Madison, WI, USA
Seth Taapken: WiCell Research Institute, Madison, WI, USA
Erich Berndt: Waisman Center, University of Wisconsin, Madison, WI, USA
Su-Chun Zhang: Waisman Center, University of Wisconsin, Madison, WI, USA; Departments of Neuroscience, Neurology, School of Medicine and Public Health, University of Wisconsin, Madison, WI, USA
Anita Bhattacharyya: Waisman Center, University of Wisconsin, Madison, WI, USA; Department of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin, Madison, WI, USA; Corresponding author at: 623 Waisman Center, 1500 Highland Avenue, Madison, WI 53705, USA.

Journal volume & issue: Vol. 34

Abstract

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Seven human induced pluripotent stem cell (iPSC) lines were generated from fibroblasts from three neonatal individuals using non-integrative reprogramming. Most control iPSCs are derived from adults, so these iPSCs meet the need for control iPSCs from young individuals. Donors were from different ethnicities and these lines provide unique genetic profiles. All iPSCs have normal karyotypes, express stem cell markers, and exhibit pluripotency, as assessed by capacity to differentiate into three germ layers. These lines are valuable to study human development, as age-matched controls for disorder-specific iPSCs, and as platforms for gene editing to control for age and ethnicity. Keywords: Neonatal, Unaffected, Control iPSCs

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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