Molecular Exploration of <i>Mycoplasma fermentans</i> and <i>Mycoplasma genitalium</i> in Mexican Women with Cervicitis
Abraham David Bustos-López,
Marcos R. Escobedo-Guerra,
Marcela López-Hurtado,
Jesús Roberto Villagrana-Zesati,
Martha Valdés-Ramírez,
Silvia Giono-Cerezo,
Fernando M. Guerra-Infante
Affiliations
Abraham David Bustos-López
Department of Microbiology, National School of Biological Sciences, National Polytechnic Institute, Prol Carpio and Plan de Ayala, Col Santo Tomás, Mexico City 11340, Mexico
Marcos R. Escobedo-Guerra
Molecular and Cellular Bioimmunology Laboratory, National Institute of Perinatology, Montes Urales 800, Col Lomas Virreyes, Mexico City 11000, Mexico
Marcela López-Hurtado
Molecular and Cellular Bioimmunology Laboratory, National Institute of Perinatology, Montes Urales 800, Col Lomas Virreyes, Mexico City 11000, Mexico
Jesús Roberto Villagrana-Zesati
Gynecology and Obstetrics Department, National Institute of Perinatology, Montes Urales 800, Col Lomas Virreyes, Mexico City 11000, Mexico
Martha Valdés-Ramírez
Gynecology and Obstetrics Department, National Institute of Perinatology, Montes Urales 800, Col Lomas Virreyes, Mexico City 11000, Mexico
Silvia Giono-Cerezo
Department of Microbiology, National School of Biological Sciences, National Polytechnic Institute, Prol Carpio and Plan de Ayala, Col Santo Tomás, Mexico City 11340, Mexico
Fernando M. Guerra-Infante
Molecular and Cellular Bioimmunology Laboratory, National Institute of Perinatology, Montes Urales 800, Col Lomas Virreyes, Mexico City 11000, Mexico
Genital Mycoplasmas are implicated in adverse pregnancy outcomes and the development of infertility. However, the role of Mycoplasma fermentans in these outcomes has not been adequately studied; therefore, its participation in these sufferings requires further investigation. This study aimed to evaluate the prevalence of M. fermentans in pregnant and non-pregnant women. End-point PCR was used to analyze two hundred and twenty-eight endocervical samples for M. hominis, M. genitalium, M. fermentans, M. pirum, Ureaplasma urealyticum, and U. parvum diagnoses. The prevalence of Mycoplasma spp. was as follows: U. parvum was found in 83 samples (36.4%), U. urealyticum in 39 instances (17.1%), M. hominis in 36 (15.7%), M. fermentans in 32 (14%), M. genitalium in 15 (6.6%), and M. pirum in 0 samples. No association was found between the Mycoplasma spp. and some infertility conditions or adverse pregnancy. However, M. fermentans and M. hominis were found to be associated with bacterial vaginosis (RR = 3.4 CI 95% 1.85–6.3, p M. fermentans and M. hominis were isolated more often in women with bacterial vaginosis, which suggests that these bacteria could contribute to the development of this pathology.
