UPLC-Q-TOF/MS-based metabonomics reveals mechanisms for Holothuria leucospilota polysaccharides (HLP)-regulated serum metabolic changes in diabetic rats

Xin Zhang; Fuqiang Zhao; Tingting Ma; Yuanping Zheng; Jun Cao; Chuan Li; Kexue Zhu

Food Chemistry: X (Oct 2023)

UPLC-Q-TOF/MS-based metabonomics reveals mechanisms for Holothuria leucospilota polysaccharides (HLP)-regulated serum metabolic changes in diabetic rats

Xin Zhang,
Fuqiang Zhao,
Tingting Ma,
Yuanping Zheng,
Jun Cao,
Chuan Li,
Kexue Zhu

Affiliations

Xin Zhang: Engineering Research Center of Utilization of Tropical Polysaccharide Resources of Ministry of Education, School of Food Science and Engineering, Hainan University, Haikou 570228, China
Fuqiang Zhao: Engineering Research Center of Utilization of Tropical Polysaccharide Resources of Ministry of Education, School of Food Science and Engineering, Hainan University, Haikou 570228, China
Tingting Ma: Engineering Research Center of Utilization of Tropical Polysaccharide Resources of Ministry of Education, School of Food Science and Engineering, Hainan University, Haikou 570228, China
Yuanping Zheng: Engineering Research Center of Utilization of Tropical Polysaccharide Resources of Ministry of Education, School of Food Science and Engineering, Hainan University, Haikou 570228, China
Jun Cao: Engineering Research Center of Utilization of Tropical Polysaccharide Resources of Ministry of Education, School of Food Science and Engineering, Hainan University, Haikou 570228, China
Chuan Li: Engineering Research Center of Utilization of Tropical Polysaccharide Resources of Ministry of Education, School of Food Science and Engineering, Hainan University, Haikou 570228, China; Collaborative Innovation Center of Provincial and Ministerial Co-construction for Marine Food Deep Processing, Dalian Polytechnic University, Dalian 116034, China; Corresponding authors at: School of Food Scienc e an d Engineering, Hainan University , Haikou 570228 , Chin a (C . Li); Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Processing Suitability and Quality Control of the Special Tropical Crops of Hainan Province, Wanning 571533, China (K . Zhu).
Kexue Zhu: Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Processing Suitability and Quality Control of the Special Tropical Crops of Hainan Province, Wanning 571533, China; Corresponding authors at: School of Food Scienc e an d Engineering, Hainan University , Haikou 570228 , Chin a (C . Li); Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Processing Suitability and Quality Control of the Special Tropical Crops of Hainan Province, Wanning 571533, China (K . Zhu).

Journal volume & issue: Vol. 19
p. 100741

Abstract

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This study aimed to use metabolomic methods to explore how Holothuria leucospilota polysaccharides (HLP) improved metabolism disorders in the liver of Goto-Kakizaki (GK) rats with spontaneous type 2 diabetes. The results showed that HLP effectively improved the metabolic disorder. Based on KEGG functional analysis, five key biomarkers associated with bile acid metabolism were detected and screened (P < 0.05). The results of serum total bile acid levels and liver damage in diabetic rats further showed the regulatory effects of HLP on bile acid metabolism. The results of bile acid-related gene expression in the liver showed that HLP inhibited liver farnesoid X Receptor - small heterodimer partner (FXR-SHP) signalling and increased the expression of bile acid synthesis genes (P < 0.05). Our results explored the underlying mechanisms by which HLP accelerated cholesterol consumption to anti-hypercholesterolemia and anti-diabetic by inhibiting liver FXR-SHP signaling. HLP's effect on bile acid regulation provides insights into treating T2DM.

Published in Food Chemistry: X

ISSN: 2590-1575 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply; Technology: Chemical technology: Food processing and manufacture
Website: https://www.journals.elsevier.com/food-chemistry-x

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