Biodetoxification of Phenolic Inhibitors from Lignocellulose Pretreatment using Kurthia huakuii LAM0618T and Subsequent Lactic Acid Fermentation
Yuejiao Xie,
Qing Hu,
Guodong Feng,
Xu Jiang,
Jinlong Hu,
Mingxiong He,
Guoquan Hu,
Shumiao Zhao,
Yunxiang Liang,
Zhiyong Ruan,
Nan Peng
Affiliations
Yuejiao Xie
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Qing Hu
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Guodong Feng
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Xu Jiang
Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, China
Jinlong Hu
State Key Laboratory of Agricultural Microbiology, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China
Mingxiong He
Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture), Biomass Energy Technology Research Centre, Biogas Institute of Ministry of Agriculture, Chengdu 610041, China
Guoquan Hu
Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture), Biomass Energy Technology Research Centre, Biogas Institute of Ministry of Agriculture, Chengdu 610041, China
Shumiao Zhao
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Yunxiang Liang
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Zhiyong Ruan
Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, China
Nan Peng
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Phenolic inhibitors generated during alkaline pretreatment of lignocellulosic biomasses significantly hinder bacterial growth and subsequent biofuel and biochemical production. Water rinsing is an efficient method for removing these compounds. Nevertheless, this method often generates a great amount of wastewater, and leads to the loss of solid fiber particles and fermentable sugars. Kurthia huakuii LAM0618T, a recently identified microorganism, was herein shown to be able to efficiently transform phenolic compounds (syringaldehyde, hydroxybenzaldehyde, and vanillin) into less toxic acids. Taking advantage of these properties, a biodetoxification method was established by inoculating K. huakuii LAM0618T into the NH3/H2O2-pretreated unwashed corn stover to degrade phenolic inhibitors and weak acids generated during the pretreatment. Subsequently, 33.47 and 17.91 g/L lactic acid was produced by Bacillus coagulans LA204 at 50 °C through simultaneous saccharification and fermentation (SSF) from 8% (w/w) of NH3/H2O2-pretreated corn stover with or without K. huakuii LAM0618T-biodetoxification, indicating biodetoxification significantly increased lactic acid titer and yield. Importantly, using 15% (w/w) of the NH3/H2O2-pretreated K. huakuii LAM0618T-biodetoxified corn stover as a substrate through fed-batch simultaneous saccharification and fermentation, high titer and high yield of lactic acid (84.49 g/L and 0.56 g/g corn stover, respectively, with a productivity of 0.88 g/L/h) were produced by Bacillus coagulans LA204. Therefore, this study reported the first study on biodetoxification of alkaline-pretreated lignocellulosic material, and this biodetoxification method could replace water rinsing for removal of phenolic inhibitors and applied in biofuel and biochemical production using the alkaline-pretreated lignocellulosic bioresources.
