Induction of Bdnf from promoter I following electroconvulsive seizures contributes to structural plasticity in neurons of the piriform cortex

Anthony D. Ramnauth; Kristen R. Maynard; Alisha S. Kardian; BaDoi N. Phan; Madhavi Tippani; Sumita Rajpurohit; John W. Hobbs; Stephanie Cerceo Page; Andrew E. Jaffe; Keri Martinowich

Brain Stimulation (Mar 2022)

Induction of Bdnf from promoter I following electroconvulsive seizures contributes to structural plasticity in neurons of the piriform cortex

Anthony D. Ramnauth,
Kristen R. Maynard,
Alisha S. Kardian,
BaDoi N. Phan,
Madhavi Tippani,
Sumita Rajpurohit,
John W. Hobbs,
Stephanie Cerceo Page,
Andrew E. Jaffe,
Keri Martinowich

Affiliations

Anthony D. Ramnauth: Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA; Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Kristen R. Maynard: Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA; Department of Psychiatry & Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Corresponding author. Lieber Institute for Brain Development, 855 North Wolfe Street, Suite 300, Baltimore, MD, 21205, USA.
Alisha S. Kardian: Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA
BaDoi N. Phan: Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA; Computational Biology Department, School of Computer Science, Carnegie Mellon University, Pittsburgh, PA, USA; Medical Scientist Training Program, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA
Madhavi Tippani: Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA
Sumita Rajpurohit: Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA
John W. Hobbs: Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA
Stephanie Cerceo Page: Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA
Andrew E. Jaffe: Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA; Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Mental Health, Johns Hopkins University, Baltimore, MD, USA; Department of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA; Department of Psychiatry & Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Keri Martinowich: Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA; Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Psychiatry & Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA

Journal volume & issue: Vol. 15, no. 2
pp. 427 – 433

Abstract

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Background: Electroconvulsive therapy (ECT) efficacy is hypothesized to depend on induction of molecular and cellular events that trigger neuronal plasticity. Investigating how electroconvulsive seizures (ECS) impact plasticity in animal models can help inform our understanding of basic mechanisms by which ECT relieves symptoms of depression. ECS-induced plasticity is associated with differential expression of unique isoforms encoding the neurotrophin, brain-derived neurotrophic factor (BDNF). Hypothesis: We hypothesized that cells expressing the Bdnf exon 1-containing isoform are important for ECS-induced structural plasticity in the piriform cortex, a highly epileptogenic region that is responsive to ECS. Methods: We selectively labeled Bdnf exon 1-expressing neurons in mouse piriform cortex using Cre recombinase dependent on GFP technology (CRE-DOG). We then quantified changes in dendrite morphology and density of Bdnf exon 1-expressing neurons. Results: Loss of promoter I-derived BDNF caused changes in spine density and morphology in Bdnf exon 1-expressing neurons following ECS. Conclusions: Promoter I-derived Bdnf is required for ECS-induced dendritic structural plasticity in Bdnf exon 1-expressing neurons.

Published in Brain Stimulation

ISSN: 1935-861X (Print); 1876-4754 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: https://www.journals.elsevier.com/brain-stimulation

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