Analyzing self-assembled spindle dynamics in fission yeast meiosis using in vivo fluorescence imaging

Abstract

Summary: Chromosome segregation in female meiosis in many metazoans is mediated by acentrosomal spindles. The analysis of the dynamics of self-assembled spindles is a challenge due to the low availability of oocytes. Here, we present a protocol for analyzing self-assembled spindle dynamics in fission yeast meiosis using in vivo fluorescence imaging. We describe steps for starter culture preparation, meiosis induction, and sample preparation. We then detail procedures for acquisition and analysis of images of self-assembled spindles.For complete details on the use and execution of this protocol, please refer to Pineda-Santaella and Fernández-Álvarez (2019)1 and Pineda-Santaella et al. (2021).2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Cell Biology
• Microscopy
• Model Organisms