Next-generation sequencing yields the complete chloroplast genome of Pleione forrestii (Orchidaceae)

Abstract

Pleione forrestii is an endangered terrestrial orchid with significant ornamental values. Here, we report the first complete chloroplast genome of P. forrestii. The circular genome was 158,759 bp in length and consisted of a pair of inverted repeats (IR 26,429 bp), which were separated by a large single-copy region (LSC 87,236 bp) and a small single copy-region (SSC 18,665 bp). It contained 135 genes (115 unique), including 87 protein-coding genes, 38 tRNAs, and 8 rRNAs. The maximum likelihood phylogenetic analysis indicated that P. forrestii was sister to P. bulbocodioides and P. formosana.

Keywords

• chloroplast genome
• phylogenetic
• illumina sequencing
• pleione forrestii