Frontiers in Cellular and Infection Microbiology (Mar 2023)
A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3
- Yiwen Guo,
- Yiwen Guo,
- Yiwen Guo,
- Yiwen Guo,
- Yun Lin,
- Yun Lin,
- Yun Lin,
- Quan Xie,
- Quan Xie,
- Quan Xie,
- Quan Xie,
- Wenyuan Zhang,
- Wenyuan Zhang,
- Wenyuan Zhang,
- Wenyuan Zhang,
- Zhenqi Xu,
- Zhenqi Xu,
- Zhenqi Xu,
- Zhenqi Xu,
- Yifei Chao,
- Yifei Chao,
- Yifei Chao,
- Yifei Chao,
- Xudong Cao,
- Xudong Cao,
- Xudong Cao,
- Xudong Cao,
- Huiru Jiang,
- Huiru Jiang,
- Huiru Jiang,
- Huiru Jiang,
- Han Li,
- Han Li,
- Han Li,
- Han Li,
- Tuofan Li,
- Tuofan Li,
- Tuofan Li,
- Tuofan Li,
- Zhimin Wan,
- Zhimin Wan,
- Zhimin Wan,
- Zhimin Wan,
- Hongxia Shao,
- Hongxia Shao,
- Hongxia Shao,
- Hongxia Shao,
- Aijian Qin,
- Aijian Qin,
- Aijian Qin,
- Aijian Qin,
- Jianqiang Ye,
- Jianqiang Ye,
- Jianqiang Ye,
- Jianqiang Ye
Affiliations
- Yiwen Guo
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China
- Yiwen Guo
- Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Yiwen Guo
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Yiwen Guo
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Yun Lin
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China
- Yun Lin
- Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Yun Lin
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Quan Xie
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China
- Quan Xie
- Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Quan Xie
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Quan Xie
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Wenyuan Zhang
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China
- Wenyuan Zhang
- Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Wenyuan Zhang
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Wenyuan Zhang
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Zhenqi Xu
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China
- Zhenqi Xu
- Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Zhenqi Xu
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Zhenqi Xu
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Yifei Chao
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China
- Yifei Chao
- Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Yifei Chao
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Yifei Chao
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Xudong Cao
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China
- Xudong Cao
- Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Xudong Cao
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Xudong Cao
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Huiru Jiang
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China
- Huiru Jiang
- Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Huiru Jiang
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Huiru Jiang
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Han Li
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China
- Han Li
- Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Han Li
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Han Li
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Tuofan Li
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China
- Tuofan Li
- Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Tuofan Li
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Tuofan Li
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Zhimin Wan
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China
- Zhimin Wan
- Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Zhimin Wan
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Zhimin Wan
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Hongxia Shao
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China
- Hongxia Shao
- Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Hongxia Shao
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Hongxia Shao
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Aijian Qin
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China
- Aijian Qin
- Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Aijian Qin
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Aijian Qin
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- Jianqiang Ye
- Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China
- Jianqiang Ye
- Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
- Jianqiang Ye
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
- Jianqiang Ye
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China
- DOI
- https://doi.org/10.3389/fcimb.2023.1177866
- Journal volume & issue
-
Vol. 13
Abstract
Recently, the highly pathogenic serotype 4 fowl adenovirus (FAdV-4) and duck adenovirus 3 (DAdV-3) were outbroken and widespread, causing substantial economic losses to the duck industry. Therefore, there is an urgent need to generate a recombinant genetic engineering vaccine candidate against both FAdV-4 and DAdV-3. In this study, a novel recombinant FAdV-4 expressing the Fiber-2 protein of DAdV-3, designated as rFAdV-4-Fiber-2/DAdV-3, was generated based on CRISPR/Cas9 and Cre-LoxP systems. Indirect immunofluorescence assay (IFA) and western blot (WB) showed that the Fiber-2 protein of DAdV-3 in rFAdV-4-Fiber-2/DAdV-3 was expressed successfully. Moreover, the growth curve revealed that rFAdV-4-Fiber-2/DAdV-3 replicated efficiently in LMH cells and even showed a stronger replication ability compared to the wild type FAdV-4. The generation of the recombinant rFAdV-4-Fiber-2/DAdV-3 provides a potential vaccine candidate against both FAdV-4 and DAdV-3.
Keywords
