Quantitative 3D analysis of complex single border cell behaviors in coordinated collective cell migration

Adam Cliffe; David P. Doupé; HsinHo Sung; Isaac Kok Hwee Lim; Kok Haur Ong; Li Cheng; Weimiao Yu

doi:10.1038/ncomms14905

Nature Communications (Apr 2017)

Quantitative 3D analysis of complex single border cell behaviors in coordinated collective cell migration

Adam Cliffe,
David P. Doupé,
HsinHo Sung,
Isaac Kok Hwee Lim,
Kok Haur Ong,
Li Cheng,
Weimiao Yu

Affiliations

Adam Cliffe: Institute of Molecule and Cell Biology (IMCB), Computational Bioimage Analysis Unit, A*STAR
David P. Doupé: Institute of Molecule and Cell Biology (IMCB), Computational Bioimage Analysis Unit, A*STAR
HsinHo Sung: Institute of Molecule and Cell Biology (IMCB), Computational Bioimage Analysis Unit, A*STAR
Isaac Kok Hwee Lim: Institute of Molecule and Cell Biology (IMCB), Computational Bioimage Analysis Unit, A*STAR
Kok Haur Ong: Institute of Molecule and Cell Biology (IMCB), Computational Bioimage Analysis Unit, A*STAR
Li Cheng: Imaging Informatics Division, Bioinformatics Institute (BII), A*STAR
Weimiao Yu: Institute of Molecule and Cell Biology (IMCB), Computational Bioimage Analysis Unit, A*STAR

DOI: https://doi.org/10.1038/ncomms14905
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 13

Abstract

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Quantifying cell behavioursin vivo is essential to understanding the mechanisms of collective cell migration. Here the authors present an image analysis toolkit, CCMToolKit, to describe and characterize various modes of coordinated cell movements accompanying collective cell migration in Drosophilaborder cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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