DYNAMICS OF FREQUENCIES OF WOLBACHIA GENOTYPES IN DROSOPHILA MELANOGASTER POPULATION FROM UMAN’ UNDER INFLUENCE OF CLIMATE FACTORS

Abstract

Aim. To investigate the potential influence of climate parameters on the rate of Wolbachia genotypes frequencies in the population of D. melanogaster from Uman’ for the last seven years. Methods. We have surveyed Wolbachia infection status for isofemale lines from Uman’ collected during summer – fall 2013 and 2015-2017 using polymerase chain reaction (PCR) with primers specific to the 16S rRNA and wsp (Wolbachia surface protein) genes. To determine wMel and wMelCS genotypes of Wolbachia, we have conducted PCR using polymorphic markers for the presence of the insertion sequence IS5 in WD1310 locus and the copy number of minisatellite repeats in VNTR-141 locus. Infection status of flies for 2011, 2012 and 2014 years was included from our previous studies. Data of climate factors (average seasonal temperature, dew point, and precipitation) for winter and summer of each year were obtained from weather database. Statistical analysis of Wolbachia genotypes-climate factors interaction was carried out in R version 3.5.0 using multiple linear model regression. Results. We have observed the decline of Wolbachia presence in Uman’ locality and prevalence of wMel bacteria variant. Moreover, our study documented the low persistence of rare wMelCS genotype through seven years in Uman’. wMelCS frequency has been driven by the combined influence of the average temperature and humidity in summer (p = 0.03662, R^2 = 0.9995). Conclusions. The climate variability affects frequency of wMelCS genotype of Wolbachia in the D. melanogaster natural population from Uman’.

Keywords

• drosophila melanogaster
• wolbachia
• wmelcs
• дрозофіла
• ендосимбіонт
• кліматичні фактори
• умань
• україна