OncoTargets and Therapy (Jun 2021)

miR-593-3p Promotes Proliferation and Invasion in Prostate Cancer Cells by Targeting ADIPOR1

  • Huang Q,
  • Peng L,
  • Sun Y,
  • Huang J,
  • Han T,
  • Li Y,
  • Peng H

Journal volume & issue
Vol. Volume 14
pp. 3729 – 3737

Abstract

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Qiang Huang,1,* Long Peng,2,* Yuxiang Sun,1 Jiayu Huang,3 Tong Han,1 Yongjie Li,1 Hui Peng1 1Department of Nephrology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510630, People’s Republic of China; 2Department of Cardiovascular Medicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510630, People’s Republic of China; 3Department of Urology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510655, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hui PengDepartment of Nephrology, The Third Affiliated Hospital of Sun Yat-sen University, 600 Tianhe Ave, Guangzhou, Guangdong, 510630, People’s Republic of ChinaTel +86 020 85252865Email [email protected]: Accumulating evidence has indicated that dysregulation of microRNAs (miRNAs) contributes to the tumorigenesis of prostate cancer (PCa). Nevertheless, the role of miR-593-3p in the development of PCa remains unclear. The objective of this study was to investigate the role and mechanisms of miR-593-3p in PCa cells.Methods: RT-PCR was used to detect the expression levels of miR-593-3p. CCK-8, colony formation, spheroid formation and transwell assays were performed to examine the proliferation, migration and invasion of C4-2, DU145 and RWPE-1 cells. And then, transcriptome sequencing, dual-luciferase reporter assay and Western blot were taken to identify the target gene and downstream mechanisms of miR-593-3p.Results: Here, we found that miR-593-3p promoted PCa cell proliferation, colony formation, spheroid formation, migration and invasion. Further mechanistic studies revealed that miR-593-3p possessed binding sites of ADIPOR1 3ʹ-UTR and played an important role in 5ʹ-AMP-activated protein kinase (AMPK) signaling pathway and epithelial–mesenchymal transition (EMT) process. In addition, the transfection of si-ADIPOR1 also enhanced the PCa cell proliferation and invasion.Conclusion: Our study provides an empirical investigation of miR-593-3p, which exerts oncogenic function through targeting ADIPOR1 in PCa cells.Keywords: miR-593-3p, ADIPOR1, PCa, proliferation, invasion

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