High-throughput phenotypic screen and transcriptional analysis identify new compounds and targets for macrophage reprogramming

Abstract

Macrophages may polarize into different states with distinct regulatory functions for inflammation. Here the authors perform high-throughput in vitro screening of a library of ~4000 compounds to identify those with specific effects on human macrophage polarization, while RNAseq helps uncover the targets and pathways mediating these effects.