Biotecnología Vegetal (Apr 2005)
Effect of the application of AIA and sucrose in the in vitro rooting of Sonate and Lambada varieties of Anthurium andraeanum Lind.
Abstract
The effect of different concentrations of indole-3-acetic acid (AIA) and sucrose Anthurium andraeanum in the varieties ‘Lambada’ and ‘Sonate’ in the enraizamiento phase during the micropropagation of this specieswas determined in this study. Nodal explants, coming from plantlets obtained in vitro, were cultivated in a liquid culture mediumMS modified, supplemented with 2.89 and 5.71 μM AIA and 30 and 40 g.l-1 (w/v) of sucrose. The length of the buds (cm), the number of roots and the length of the roots (cm)were the evaluated variables . In the rooting phase the best sucrose concentration in the cultivation medium was of 40 g.l-1, being observed that the increment in its concentration improved the length of the plants, as well as the number and length of the roots for the two varieties. There were differences in the requirement of AIA in the evaluated variables in both varieties. Key words: Araceae, in vitro culture, micropropagation Abbreviations: IAA (Indole-3-acetic acid), NAA (naphthalenacetic acid), IBA (Indole-3-butyric acid), Kin (6-furfurylaminopurine)