State Key Lab of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, China; College of Marine Life Sciences, Ocean University of China, Qingdao, China
Xiu-Juan Wang
State Key Lab of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, China
Xiu-Lan Chen
State Key Lab of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China
Qi Sheng
State Key Lab of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, China
Shan Zhang
State Key Lab of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, China
Peng Wang
College of Marine Life Sciences, Ocean University of China, Qingdao, China
Mussa Quareshy
School of Life Sciences, University of Warwick, Coventry, United Kingdom
Branko Rihtman
School of Life Sciences, University of Warwick, Coventry, United Kingdom
Xuan Shao
State Key Lab of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, China
Chao Gao
State Key Lab of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, China
Fuchuan Li
National Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Qingdao, China
Shengying Li
State Key Lab of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, China
Weipeng Zhang
College of Marine Life Sciences, Ocean University of China, Qingdao, China
Xiao-Hua Zhang
College of Marine Life Sciences, Ocean University of China, Qingdao, China
Gui-Peng Yang
Frontiers Science Center for Deep Ocean Multispheres and Earth System, Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, China
Jonathan D Todd
School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, United Kingdom
College of Marine Life Sciences, Ocean University of China, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China; Marine Biotechnology Research Center, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China
Dimethylsulfoniopropionate (DMSP) is an abundant and ubiquitous organosulfur molecule in marine environments with important roles in global sulfur and nutrient cycling. Diverse DMSP lyases in some algae, bacteria, and fungi cleave DMSP to yield gaseous dimethyl sulfide (DMS), an infochemical with important roles in atmospheric chemistry. Here, we identified a novel ATP-dependent DMSP lyase, DddX. DddX belongs to the acyl-CoA synthetase superfamily and is distinct from the eight other known DMSP lyases. DddX catalyses the conversion of DMSP to DMS via a two-step reaction: the ligation of DMSP with CoA to form the intermediate DMSP-CoA, which is then cleaved to DMS and acryloyl-CoA. The novel catalytic mechanism was elucidated by structural and biochemical analyses. DddX is found in several Alphaproteobacteria, Gammaproteobacteria, and Firmicutes, suggesting that this new DMSP lyase may play an overlooked role in DMSP/DMS cycles.
