Data set for the proteomic inventory and quantitative analysis of chicken eggshell matrix proteins during the primary events of eggshell mineralization and the active growth phase of calcification
Pauline Marie,
Valérie Labas,
Aurélien Brionne,
Grégoire Harichaux,
Christelle Hennequet-Antier,
Alejandro B. Rodriguez-Navarro,
Yves Nys,
Joël Gautron
Affiliations
Pauline Marie
INRA, UR83 Recherches avicoles, Fonction et Régulation des protéines de l׳œuf, F-37380 Nouzilly, France
Valérie Labas
INRA, UMR INRA85, UMR CNRS 7247; Université de Tours; IFCE, Physiologie de la Reproduction et des Comportements, Plate-forme d׳Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly, France
Aurélien Brionne
INRA, UR83 Recherches avicoles, Fonction et Régulation des protéines de l׳œuf, F-37380 Nouzilly, France
Grégoire Harichaux
INRA, UMR INRA85, UMR CNRS 7247; Université de Tours; IFCE, Physiologie de la Reproduction et des Comportements, Plate-forme d׳Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly, France
Christelle Hennequet-Antier
INRA, UR83 Recherches avicoles, Fonction et Régulation des protéines de l׳œuf, F-37380 Nouzilly, France
Alejandro B. Rodriguez-Navarro
Departmento de Mineralogia y Petrologia, Universidad de Granada, Campus Fuente Nueva s/n, 18002 Granada, Spain
Yves Nys
INRA, UR83 Recherches avicoles, Fonction et Régulation des protéines de l׳œuf, F-37380 Nouzilly, France
Joël Gautron
INRA, UR83 Recherches avicoles, Fonction et Régulation des protéines de l׳œuf, F-37380 Nouzilly, France
Chicken eggshell is a biomineral composed of 95% calcite calcium carbonate mineral and of 3.5% organic matrix proteins. The assembly of mineral and its structural organization is controlled by its organic matrix. In a recent study [1], we have used quantitative proteomic, bioinformatic and functional analyses to explore the distribution of 216 eggshell matrix proteins at four key stages of shell mineralization defined as: (1) widespread deposition of amorphous calcium carbonate (ACC), (2) ACC transformation into crystalline calcite aggregates, (3) formation of larger calcite crystal units and (4) rapid growth of calcite as columnar structure with preferential crystal orientation. The current article detailed the quantitative analysis performed at the four stages of shell mineralization to determine the proteins which are the most abundant. Additionally, we reported the enriched GO terms and described the presence of 35 antimicrobial proteins equally distributed at all stages to keep the egg free of bacteria and of 81 proteins, the function of which could not be ascribed.
