Generation of green fluorescent protein reporter knock-in iPSC line at the 3′UTR region of the KLOTHO locus

Sun Woo Lim; Kang In Lee; Sheng Cui; Xianying Fang; Yoo Jin Shin; Hanbi Lee; Jae Young Lee; Byung Ha Chung; Chul Woo Yang

Stem Cell Research (Oct 2024)

Generation of green fluorescent protein reporter knock-in iPSC line at the 3′UTR region of the KLOTHO locus

Sun Woo Lim,
Kang In Lee,
Sheng Cui,
Xianying Fang,
Yoo Jin Shin,
Hanbi Lee,
Jae Young Lee,
Byung Ha Chung,
Chul Woo Yang

Affiliations

Sun Woo Lim: Transplantation Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea
Kang In Lee: R&D Center, ToolGen, Inc., Seoul, Republic of Korea
Sheng Cui: Transplantation Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea
Xianying Fang: Transplantation Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea
Yoo Jin Shin: Transplantation Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea
Hanbi Lee: Transplantation Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Internal Medicine, Seoul St. Mary’s Hospital, The Catholic University of Korea, Seoul, Republic of Korea
Jae Young Lee: R&D Center, ToolGen, Inc., Seoul, Republic of Korea
Byung Ha Chung: Transplantation Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Internal Medicine, Seoul St. Mary’s Hospital, The Catholic University of Korea, Seoul, Republic of Korea; Corresponding authors at: Transplantation Research Centre & Division of Nephrology, Department of Internal Medicine, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of Korea.
Chul Woo Yang: Transplantation Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Internal Medicine, Seoul St. Mary’s Hospital, The Catholic University of Korea, Seoul, Republic of Korea; Corresponding authors at: Transplantation Research Centre & Division of Nephrology, Department of Internal Medicine, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of Korea.

Journal volume & issue: Vol. 80
p. 103499

Abstract

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We generated a human induced pluripotent stem cell (hiPSC) line (CMCi014-A-78) expressing a GFP reporter in the 3′-UTR region of the KLOTHO locus using CRISPR/Cas9-mediated homologous recombination to screen for candidates regulating KLOTHO. The established cell line exhibits a normal karyotype, typical stem cell morphology, expression of pluripotency markers, and the ability to differentiate into the three germ layers. Consequently, this hiPSC line could serve as a valuable resource for screening KLOTHO regulators in hiPSC-derived target cells or organoids.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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