Establishment of an induced pluripotent stem cell line (JTUi003-A) from a patient with otosclerosis

Yuanping Xiong; Ying Wang; Maoxiang Xu; Yaoqian Liu; Hongqun Jiang; Zhuang Jiang; Jiping Wang

Stem Cell Research (Apr 2022)

Establishment of an induced pluripotent stem cell line (JTUi003-A) from a patient with otosclerosis

Yuanping Xiong,
Ying Wang,
Maoxiang Xu,
Yaoqian Liu,
Hongqun Jiang,
Zhuang Jiang,
Jiping Wang

Affiliations

Yuanping Xiong: Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital of Nanchang University, Nanchang 330006, China
Ying Wang: Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China; Otolaryngology Institute of Shanghai Jiao Tong University, Shanghai 200233, China
Maoxiang Xu: Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China; Otolaryngology Institute of Shanghai Jiao Tong University, Shanghai 200233, China
Yaoqian Liu: Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China; Otolaryngology Institute of Shanghai Jiao Tong University, Shanghai 200233, China
Hongqun Jiang: Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital of Nanchang University, Nanchang 330006, China
Zhuang Jiang: Department of Otolaryngology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China; Corresponding authors at: Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China (J. Wang) and Department of Otolaryngology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, People's Republic of China (Z. Jiang).
Jiping Wang: Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China; Otolaryngology Institute of Shanghai Jiao Tong University, Shanghai 200233, China; Shanghai Key Laboratory of Sleep Disordered Breathing, Shanghai 200233, China; Corresponding authors at: Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China (J. Wang) and Department of Otolaryngology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, People's Republic of China (Z. Jiang).

Journal volume & issue: Vol. 60
p. 102666

Abstract

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Otosclerosis is characterized by abnormal bone remodeling in the osseous labyrinth and progressive hearing loss. Although the etiology of otosclerosis is not fully understood, both environmental and genetic factors play important roles in its pathogenesis. Here, we generated an induced pluripotent stem cell line using episomal plasmid vectors from the peripheral blood mononuclear cells of a 48-year-old male with otosclerosis. The morphology and karyotype of the cells were normal. The expression of pluripotency markers was verified by mRNA and protein levels; the pluripotency state of the cell line was verified by successful differentiation into all three germ layers.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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