Amylase-producing marine actinobacterium of Micromonospora sp. and their potential antibacterial effects against oral pathogens

Abstract

Marine actinobacteriological investigation is still in its beginning in India. Earlier, in the 20th century, studies on Actinobacteria were started and highly concentrated on diversity, identification, and screening for enzymes, antibiotics, and enzyme inhibitors. With the spurge of infectious diseases requiring antibiotics, novel antibiotics are in search as the prevalent ones have declined uses, due to the antibiotic-resistant microbial growth. Unexploited ecosystems are studied for isolation of rare species such as Actinobacteria which are expected to yield newer metabolites. The marine actinobacterial isolation and enumeration were done from sediment samples. The marine Actinobacteria were identified by conventional methods. Further amylase enzyme production and their antibacterial activities are also done following the standard methods. The Micromonospora sp. was identified by chemotaxonomical characteristics and spore chain morphology. Further, the amylase enzyme production was done and quantification of enzyme also done. The potential antimicrobial activity from the amylase enzyme was done. Zone of inhibition and minimal inhibitory concentration were calculated. It concluded that potent antibacterial activity was obtained from Actinobacteria Micromonospora sp. producing amylase enzymes.

Keywords

• antibacterial activity
• marine actinobacteria
• minimal inhibitory concentration
• micromonospora sp. novel enzyme