Nature Communications (Jun 2018)
Conformational switching of the pseudokinase domain promotes human MLKL tetramerization and cell death by necroptosis
- Emma J. Petrie,
- Jarrod J. Sandow,
- Annette V. Jacobsen,
- Brian J. Smith,
- Michael D. W. Griffin,
- Isabelle S. Lucet,
- Weiwen Dai,
- Samuel N. Young,
- Maria C. Tanzer,
- Ahmad Wardak,
- Lung-Yu Liang,
- Angus D. Cowan,
- Joanne M. Hildebrand,
- Wilhelmus J. A. Kersten,
- Guillaume Lessene,
- John Silke,
- Peter E. Czabotar,
- Andrew I. Webb,
- James M. Murphy
Affiliations
- Emma J. Petrie
- The Walter & Eliza Hall Institute of Medical Research
- Jarrod J. Sandow
- The Walter & Eliza Hall Institute of Medical Research
- Annette V. Jacobsen
- The Walter & Eliza Hall Institute of Medical Research
- Brian J. Smith
- Department of Biochemistry & Molecular Biology, LaTrobe University
- Michael D. W. Griffin
- Department of Biochemistry & Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, The Bio21 Institute
- Isabelle S. Lucet
- The Walter & Eliza Hall Institute of Medical Research
- Weiwen Dai
- The Walter & Eliza Hall Institute of Medical Research
- Samuel N. Young
- The Walter & Eliza Hall Institute of Medical Research
- Maria C. Tanzer
- The Walter & Eliza Hall Institute of Medical Research
- Ahmad Wardak
- The Walter & Eliza Hall Institute of Medical Research
- Lung-Yu Liang
- The Walter & Eliza Hall Institute of Medical Research
- Angus D. Cowan
- The Walter & Eliza Hall Institute of Medical Research
- Joanne M. Hildebrand
- The Walter & Eliza Hall Institute of Medical Research
- Wilhelmus J. A. Kersten
- The Walter & Eliza Hall Institute of Medical Research
- Guillaume Lessene
- The Walter & Eliza Hall Institute of Medical Research
- John Silke
- The Walter & Eliza Hall Institute of Medical Research
- Peter E. Czabotar
- The Walter & Eliza Hall Institute of Medical Research
- Andrew I. Webb
- The Walter & Eliza Hall Institute of Medical Research
- James M. Murphy
- The Walter & Eliza Hall Institute of Medical Research
- DOI
- https://doi.org/10.1038/s41467-018-04714-7
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 15
Abstract
RIPK3-mediated phosphorylation of the mixed lineage kinase domain-like (MLKL) pseudokinase is thought to be the trigger for MLKL activation during necroptotic signaling. Here the authors provide evidence that the transition of human MLKL from a monomeric state to a tetramer is essential for necroptosis signalling.
