Improving the Technological Cycle of Microclonal Propagation of Rubus chamaemorus L.

Abstract

The results of the study of the microclonal propagation of the cloudberry (Rubus chamaemorus L.) of the Leningradskaya and Kondinskaya forms at the stages of microclonal propagation itself and rooting of microshoots in in vitro culture are presented. R. chamaemorus is one of the most popular bog berry plants in the countries of Northern Europe and the northern regions of Russia, possessing highly valuable nutritional and pharmacological properties. In order to intensify industrial berry growing in Russia and meet the market demand for berry products in the context of import substitution, it is necessary to use high-tech methods for obtaining planting material. In order to preserve the valuable gene pool and accelerate the production of a large amount of healthy planting material of R. chamaemorus forms, it is necessary to improve and optimize the technologies or microclonal propagation of this species. The largest number (on average 9.6–9.9 pcs.) and the total length (16.4– 19.5 cm) of R. chamaemorus microshoots in in vitro culture at the stage of microclonal propagation itself have been observed on the Murashige and Skoog culture medium. An increase in the concentration of the “Dropp” preparation from 0.1 to 0.2 mg/l in the culture medium has contributed to an increase in the number of R. chamaemorus microshoots (on average by 1.8–2.4 times), an increase in their total length in the Konsinskaya form (by 1.5 times) and its decrease in the Leningradskaya form (by 1.1 times). The largest number (on average 3.9–4.6 pcs.) and the total length (13.2–14.0 cm) of R. chamaemorus roots at the stage of microshoot rooting in vitro have been noted on the Murashige and Skoog culture medium. An increase in the concentration of indolebutyric acid from 0.5 to 1.0 mg/l in the culture medium has contributed to an increase in the number of R. chamaemorus roots (on average by 1.4 times) and a decrease in their total length (by 1.15–1.25 times).

Keywords

• cloudberry
• forest berry plants
• in vitro
• growth regulators
• culture medium
• microclonal propagation