Immunoprecipitation of Proteins in Caenorhabditis elegans
Kevin Chan,
Ashwin Seetharaman,
Guillermo Selman,
Peter Roy
Affiliations
Kevin Chan
Department of Molecular Genetics, University of Toronto, Toronto, CanadaThe Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Canada
Ashwin Seetharaman
Department of Molecular Genetics, University of Toronto, Toronto, CanadaThe Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Canada, The Collaborative Programme in Developmental Biology, University of Toronto, Toronto, Canada
Guillermo Selman
Department of Molecular Genetics, University of Toronto, Toronto, CanadaThe Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Canada
Peter Roy
Department of Molecular Genetics, University of Toronto, Toronto, CanadaThe Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Canada, The Collaborative Programme in Developmental Biology, University of Toronto, Toronto, Canada
Immunoprecipitation (IP) is a biochemical technique to precipitate a protein out of solution using an antigen that can specifically bind to that protein. IP can be performed to isolate and concentrate one particular protein from a sample of thousands of different proteins. IP is also readily performed to pull down interacting proteins of complexes out of solution. This protocol outlines the methods used to IP proteins in whole worm lysates and their preparation for detection on Western blots using denaturing conditions.
