Bioinformatics Prediction and in vivo Verification Identify SLC7A5 as Immune Infiltration Related Biomarker in Breast Cancer

Abstract

Qiannan Zhu,1 Jue Wang,1 Yuenian Shi,2 Xiaoming Zha,1 Shui Wang1 1The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, People’s Republic of China; 2Nanjing Medical University, Nanjing, Jiangsu, 210029, People’s Republic of ChinaCorrespondence: Shui Wang; Xiaoming Zha, The First Affiliated Hospital of Nanjing Medical University, 300# Guangzhou Road, Nanjing, 210000, People’s Republic of China, Email [email protected]; [email protected]: Solute carrier family 7, member 5 (SLC7A5) is reportedly a key gene in various tumors. However, the relationship between SLC7A5 and immune cell infiltration in breast cancer remains elusive. In this study, we investigated the expression levels and clinical value of SLC7A5 using the R package in breast cancer through the TCGA database.Methods: Public transcript data and clinical information of patients were downloaded from the TCGA database. Prognosis analysis was performed to explore the clinical value of SLC7A5 as well as drug sensitivity prediction and Gene Set Enrichment Analysis (GSEA). We also explored the correlations between SLC7A5 and immune infiltration as well as immune markers.Results: We observed that high SLC7A5 expression levels were related to poor survival. Our GSEA demonstrated that G protein-coupled receptor-ligand binding, interleukin signaling, neutrophil degranulation, amino acid and derivative metabolism, and Rho GTPase signaling were differentially enriched in the SLC7A5 high-expression phenotype. Patients in the low SLC7A5 expression group showed sensitivity to paclitaxel and doxorubicin.Conclusion: SLC7A5 could serve as a biomarker for breast cancer prognosis.Keywords: SLC7A5, breast cancer, immune cell infiltration, chemosensitivity

Keywords

• slc7a5
• breast cancer
• immune cell infiltration
• chemosensitivity