Nature Communications (Feb 2021)
Combinatorial CRISPR screen identifies fitness effects of gene paralogues
- Nicola A. Thompson,
- Marco Ranzani,
- Louise van der Weyden,
- Vivek Iyer,
- Victoria Offord,
- Alastair Droop,
- Fiona Behan,
- Emanuel Gonçalves,
- Anneliese Speak,
- Francesco Iorio,
- James Hewinson,
- Victoria Harle,
- Holly Robertson,
- Elizabeth Anderson,
- Beiyuan Fu,
- Fengtang Yang,
- Guido Zagnoli-Vieira,
- Phil Chapman,
- Martin Del Castillo Velasco-Herrera,
- Mathew J. Garnett,
- Stephen P. Jackson,
- David J. Adams
Affiliations
- Nicola A. Thompson
- Wellcome Sanger Institute, Wellcome Trust Genome Campus
- Marco Ranzani
- Wellcome Sanger Institute, Wellcome Trust Genome Campus
- Louise van der Weyden
- Wellcome Sanger Institute, Wellcome Trust Genome Campus
- Vivek Iyer
- Wellcome Sanger Institute, Wellcome Trust Genome Campus
- Victoria Offord
- Wellcome Sanger Institute, Wellcome Trust Genome Campus
- Alastair Droop
- Wellcome Sanger Institute, Wellcome Trust Genome Campus
- Fiona Behan
- Wellcome Sanger Institute, Wellcome Trust Genome Campus
- Emanuel Gonçalves
- Wellcome Sanger Institute, Wellcome Trust Genome Campus
- Anneliese Speak
- Wellcome Sanger Institute, Wellcome Trust Genome Campus
- Francesco Iorio
- Wellcome Sanger Institute, Wellcome Trust Genome Campus
- James Hewinson
- Wellcome Sanger Institute, Wellcome Trust Genome Campus
- Victoria Harle
- Wellcome Sanger Institute, Wellcome Trust Genome Campus
- Holly Robertson
- Wellcome Sanger Institute, Wellcome Trust Genome Campus
- Elizabeth Anderson
- Wellcome Sanger Institute, Wellcome Trust Genome Campus
- Beiyuan Fu
- Wellcome Sanger Institute, Wellcome Trust Genome Campus
- Fengtang Yang
- Wellcome Sanger Institute, Wellcome Trust Genome Campus
- Guido Zagnoli-Vieira
- Wellcome Trust/Cancer Research UK Gurdon Institute
- Phil Chapman
- Cancer Research UK, Manchester Institute
- Martin Del Castillo Velasco-Herrera
- Wellcome Sanger Institute, Wellcome Trust Genome Campus
- Mathew J. Garnett
- Wellcome Sanger Institute, Wellcome Trust Genome Campus
- Stephen P. Jackson
- Wellcome Trust/Cancer Research UK Gurdon Institute
- David J. Adams
- Wellcome Sanger Institute, Wellcome Trust Genome Campus
- DOI
- https://doi.org/10.1038/s41467-021-21478-9
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 11
Abstract
Systematic screens for synthetic lethal gene pairs represent a powerful approach to define interactions that may be exploited in the clinic. Here, the authors use a dual-guide CRISPR screening approach to screen a curated selection of gene pairs across three cell lines and validate the Fam50a/Fam50b synthetic lethality was in isogenic cell models as well as in an in vivo setting.
