Comprehensive Proteomics Analysis of Function Study of AKR4C14, an Aldo-keto Reductase from Thai Jasmine Rice by Applying NGS with Biopython

Abstract

Aldo-keto reductases (AKRs) are important enzymes involved in various metabolic pathways, including the detoxification of aldehydes and ketones.AKR4C14, which is an aldo-keto reductase found in Thai Jasmine rice (Oryza sativa), has been linked to crucial physiological processes. However, its functional roles have not been extensively studied. In this research, we will use next-generation sequencing (NGS) technologies along with biopython tools to conduct a thorough proteomics analysis of AKR4C14. Aldo-keto reductases (AKRs) are oxidoreductase enzymes that metabolize aldehydes and ketones into their corresponding alcohols in a NADPH/NADP+ dependent manner. Without the cofactor, the apo structure of indica rice AKR4C14 was likewise determined, displaying the stabilized open conformation. Our goal is to understand the functional roles of AKR4C14 and how it interacts with metabolic pathways in rice by integrating proteomic data.

Keywords

• Oryza sativa, AKR4C14, Next-Generation Sequencing, Biopython, Proteomics