Infection and Drug Resistance (2020-09-01)

Emergence of ST15 Klebsiella pneumoniae Clinical Isolates Producing Plasmids-Mediated RmtF and OXA-232 in China

  • Shi Q,
  • Han R,
  • Guo Y,
  • Zheng Y,
  • Yang Y,
  • Yin D,
  • Zhang R,
  • Hu F

Journal volume & issue
Vol. Volume 13
pp. 3125 – 3129


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Qingyu Shi,1,2 Renru Han,1,2 Yan Guo,1,2 Yonggui Zheng,1,2 Yang Yang,1,2 Dandan Yin,1,2 Rong Zhang,3 Fupin Hu1,2 1Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, People’s Republic of China; 2Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai, People’s Republic of China; 3Department of Clinical Laboratory Medicine, Second Affiliated Hospital of Zhejiang University, Hangzhou, People’s Republic of ChinaCorrespondence: Fupin Hu Email [email protected]: RmtF, as 16S rRNA methyltransferase, leads to high-level resistance to aminoglycoside and is now barely reported.Methods and Results: Three rmtF-positive Klebsiella pneumoniae isolates, belonging to the pandemic clone sequence type 15, were isolated from children and coproduced blaOXA-232 and blaCTX-M-15. The rmtF gene was located on an IncFIB transformable plasmid of 128,536-bp and blaOXA-232 was on a 6141-bp ColKP3 plasmid, respectively.Conclusion: Plasmids with rmtF found worldwide, shared relatively low similarity, and merely matched partly in their multidrug resistance region. Notably, clinical isolates coproducing rmtF and blaOXA-232 are gradually increasing in China.Keywords: Klebsiella pneumoniae, RmtF, blaOXA-232, ST15, aminoglycoside