Investigation on the Protective Role of Nitric Oxide in Reducing Damages Induced by Salinity Stress in Calendula officinalis L.

Majallah-i ̒Ulum-i Bāghbānī. 2017;30(2):185-191 DOI 10.22067/jhorts4.v30i2.35847


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Journal Title: Majallah-i ̒Ulum-i Bāghbānī

ISSN: 2008-4730 (Print); 2423-3986 (Online)

Publisher: Ferdowsi University of Mashhad

LCC Subject Category: Agriculture: Agriculture (General)

Country of publisher: Iran, Islamic Republic of

Language of fulltext: Persian

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maryam jabbarzadeh (Ferdowsi University of Mashhad)
َAli Tehranifar (Ferdowsi University of Mashhad)
Jafar Amiri (Urmia University)
Bahram Abedy (Ferdowsi University of Mashhad)


Double blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 24 weeks


Abstract | Full Text

Introduction: Salinity is one of the most important environmental factors that regulates plant growth and development, and limits plant production. Researchers have shown that some plant growth regulators such as nitric oxide improve the plants resistance to environmental stresses such as heat, cold, drought and salinity. Sodium nitroprusside (SNP) commonly has been used as nitric oxide (NO) donor in plants. NO is a diffusible gaseous free radical. Low concentrations of NO inhibit the production of reactive oxygen species and protect plants against ROS damages. The aim of this study was to evaluate the role of SNP as NO donor on salt tolerance of Calendula officinalis and its effects on some morphological, physiological and biochemical characteristics of this plant. Materials and Methods: In this study, the effects of salinity (0, 25, 50, 75 and 100 mM) and sodium nitroprusside (0.0, 0.25, 0.50 and 0.75 mM) on morphological and physiological characteristics of Calendula officinalis L. were investigated. Total leaf area and number of leaves were determined in the end of the experiment. Electrolyte leakage was used to asses’ membrane permeability. This procedure was based on Lutts et al.,1995. Soluble sugars were extracted and estimated by the method of Irigoyen et al., 1992. Chlorophyll a, b and carotenoid content were calculated from the absorbance of extract at 653, 666 and 470 nm using the formula of Dere et al., 1998. Proline was extracted by the method of Bates et al., 1973. DPPH radical- scavenging activity of sample was performed as described previously of Cleep et al., 2012. The SAS software was used for the analysis of variance (ANOVA), comparisons with P