Resistance training mitigates hepato-cardiac changes and muscle mitochondrial protein reductions in rats with diet-induced obesity
Caroline Pancera Laurindo,
Karen C. Rego Gregorio,
Ana Caroline Rippi Moreno,
Julia Maia Viudes Agostinho,
Evelyn Carvalho Campos,
Gisele Alborghetti Nai,
Maria Tereza Nunes,
Patrícia Monteiro Seraphim
Affiliations
Caroline Pancera Laurindo
Department of Physiotherapy - School of Sciences and Technology - Sao Paulo, State University - UNESP, Campus Presidente Prudente, Brazil
Karen C. Rego Gregorio
Department of Physiotherapy - School of Sciences and Technology - Sao Paulo, State University - UNESP, Campus Presidente Prudente, Brazil
Ana Caroline Rippi Moreno
Department of Physiotherapy - School of Sciences and Technology - Sao Paulo, State University - UNESP, Campus Presidente Prudente, Brazil; Department of Physiology and Biophysics – Institute of Biomedical Sciences I, University of Sao Paulo, Sao Paulo, SP, Brazil
Julia Maia Viudes Agostinho
Department of Physiotherapy - School of Sciences and Technology - Sao Paulo, State University - UNESP, Campus Presidente Prudente, Brazil
Evelyn Carvalho Campos
Department of Physiotherapy - School of Sciences and Technology - Sao Paulo, State University - UNESP, Campus Presidente Prudente, Brazil
Gisele Alborghetti Nai
Department of Pathology - University of Western Sao Paulo, Presidente Prudente, SP, Brazil
Maria Tereza Nunes
Department of Physiology and Biophysics – Institute of Biomedical Sciences I, University of Sao Paulo, Sao Paulo, SP, Brazil
Patrícia Monteiro Seraphim
Department of Physiotherapy - School of Sciences and Technology - Sao Paulo, State University - UNESP, Campus Presidente Prudente, Brazil; Corresponding author.
Aim: To investigate the effect of resistance training (RT) on hepatocardiovascular and muscle mitochondrial parameters in rats that were fed a high-calorie diet for 12 weeks. Main methods: The animals were divided into four groups: control (C), exercise (E), obese (O), and obese plus exercise (OE). Group E and OE rats performed resistance training by climbing on a vertical ladder with load attached to the end of the tail (1×/day, 3×/week, for 12 weeks). Group O and OE rats were fed a high-calorie diet containing chow and a cafeteria diet for 12 weeks. Under anesthesia, the heart and liver were removed for histopathological analysis, and the gastrocnemius muscle was removed for Western blotting. Key findings: Group O rats were heavier, with increased fat mass, elevated fasting glycemia, and total triglycerides, and exhibited a significant number of Kupffer cells and diffuse steatosis in the liver. Group O rats also showed increased thickness of the right ventricle, septum, and pulmonary artery. All of these parameters were attenuated by RT. PGC1-α protein levels were increased in both exercise groups. The protein levels of OXPHOS complexes III, IV, and V were reduced in Group O, while RT prevented this alteration. Significance: RT exerts a protective effect against hepato-cardiac alterations and prevents changes in the muscle mitochondrial protein profile induced by a high-calorie diet.
