Impact of sterilization methods on dissolved trace metals concentrations in complex natural samples: Optimization of UV irradiation

Abstract

Sterilization is essential for discriminating biotic responses from abiotic reactions in laboratory experiments investigating biogeochemical processes of complex natural samples. However, the conventional methods used to effectively sterilize materials or culture media do not allow sterilizing complex natural samples while maintaining biogeochemical balances. The aim of this study was to develop a low-cost and easy-to-use method to obtain geochemically unmodified and sterilized samples from complex lacustrine or coastal marine ecosystems. In preliminary assays, the impact of several sterilization methods (autoclaving, chemical poisoning, microwave, UV irradiation) on the trace metals balances was studied using borosilicate glass (BG), fluorinated ethylene-propylene (FEP) or polyethylene terephthalate (PET) bottles. Unlike other methods, UV sterilization had minor effects on the distribution of dissolved trace metals. Additional tests using complex lacustrine and coastal marine samples under 10 g/L sediments were performed using a homemade UV sterilization chamber designed to simultaneously irradiate a large number samples. Results showed: • very reproducible UV tests in BG and FEP bottles • faster sterilization using FEP bottles than using BG bottles • low variations of dissolved trace metals concentrations, except for Al, Cu, Fe and Zn Method name: UV sterilization of complex natural (lacustrine or coastal marine) samples, Keywords: UV, Sterilization, Trace metals, Natural ecosystems, Complex matrices