Nature Communications (Jun 2021)
Robust detection of translocations in lymphoma FFPE samples using targeted locus capture-based sequencing
- Amin Allahyar,
- Mark Pieterse,
- Joost Swennenhuis,
- G. Tjitske Los-de Vries,
- Mehmet Yilmaz,
- Roos Leguit,
- Ruud W. J. Meijers,
- Robert van der Geize,
- Joost Vermaat,
- Arjen Cleven,
- Tom van Wezel,
- Arjan Diepstra,
- Léon C. van Kempen,
- Nathalie J. Hijmering,
- Phylicia Stathi,
- Milan Sharma,
- Adrien S. J. Melquiond,
- Paula J. P. de Vree,
- Marjon J. A. M. Verstegen,
- Peter H. L. Krijger,
- Karima Hajo,
- Marieke Simonis,
- Agata Rakszewska,
- Max van Min,
- Daphne de Jong,
- Bauke Ylstra,
- Harma Feitsma,
- Erik Splinter,
- Wouter de Laat
Affiliations
- Amin Allahyar
- Oncode Institute & Hubrecht Institute-KNAW and University Medical Center Utrecht
- Mark Pieterse
- Oncode Institute & Hubrecht Institute-KNAW and University Medical Center Utrecht
- Joost Swennenhuis
- Cergentis BV
- G. Tjitske Los-de Vries
- Amsterdam UMC-Vrije Universiteit Amsterdam, Department of Pathology and Cancer Center Amsterdam
- Mehmet Yilmaz
- Cergentis BV
- Roos Leguit
- University Medical Centre Utrecht, Department of Pathology
- Ruud W. J. Meijers
- University Medical Centre Utrecht, Department of Pathology
- Robert van der Geize
- Laboratorium Pathologie Oost-Nederland
- Joost Vermaat
- Leiden University Medical Centre, Department of Hematology
- Arjen Cleven
- Leiden University Medical Center, Department of Pathology
- Tom van Wezel
- Leiden University Medical Center, Department of Pathology
- Arjan Diepstra
- University of Groningen, University Medical Centre Groningen, Department of Pathology & Medical Biology
- Léon C. van Kempen
- University of Groningen, University Medical Centre Groningen, Department of Pathology & Medical Biology
- Nathalie J. Hijmering
- Amsterdam UMC-Vrije Universiteit Amsterdam, Department of Pathology and Cancer Center Amsterdam
- Phylicia Stathi
- Amsterdam UMC-Vrije Universiteit Amsterdam, Department of Pathology and Cancer Center Amsterdam
- Milan Sharma
- Oncode Institute & Hubrecht Institute-KNAW and University Medical Center Utrecht
- Adrien S. J. Melquiond
- Oncode Institute & Hubrecht Institute-KNAW and University Medical Center Utrecht
- Paula J. P. de Vree
- Oncode Institute & Hubrecht Institute-KNAW and University Medical Center Utrecht
- Marjon J. A. M. Verstegen
- Oncode Institute & Hubrecht Institute-KNAW and University Medical Center Utrecht
- Peter H. L. Krijger
- Oncode Institute & Hubrecht Institute-KNAW and University Medical Center Utrecht
- Karima Hajo
- Cergentis BV
- Marieke Simonis
- Cergentis BV
- Agata Rakszewska
- Cergentis BV
- Max van Min
- Cergentis BV
- Daphne de Jong
- Amsterdam UMC-Vrije Universiteit Amsterdam, Department of Pathology and Cancer Center Amsterdam
- Bauke Ylstra
- Amsterdam UMC-Vrije Universiteit Amsterdam, Department of Pathology and Cancer Center Amsterdam
- Harma Feitsma
- Cergentis BV
- Erik Splinter
- Cergentis BV
- Wouter de Laat
- Oncode Institute & Hubrecht Institute-KNAW and University Medical Center Utrecht
- DOI
- https://doi.org/10.1038/s41467-021-23695-8
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 15
Abstract
Preservation of cancer biopsies by FFPE introduces DNA fragmentation, hindering analysis of rearrangements. Here the authors introduce FFPE Targeted Locus Capture for identification of translocations in preserved samples.
